Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   GFD20_RS19355 Genome accession   NZ_CP045768
Coordinates   4224175..4225512 (+) Length   445 a.a.
NCBI ID   WP_003110427.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CFSAN084950     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 4219175..4230512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFD20_RS19330 (GFD20_19330) pgeF 4219318..4220046 (-) 729 WP_003102588.1 peptidoglycan editing factor PgeF -
  GFD20_RS19335 (GFD20_19335) rluD 4220043..4221005 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GFD20_RS19340 (GFD20_19340) - 4221151..4222176 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  GFD20_RS19345 (GFD20_19345) - 4222333..4222578 (+) 246 WP_003102591.1 PP0621 family protein -
  GFD20_RS19350 (GFD20_19350) pilS 4222568..4224160 (+) 1593 WP_003094692.1 two-component system sensor histidine kinase PilS Regulator
  GFD20_RS19355 (GFD20_19355) pilR 4224175..4225512 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  GFD20_RS19360 (GFD20_19360) thiO 4225558..4226652 (-) 1095 WP_003145981.1 glycine oxidase ThiO -
  GFD20_RS19365 (GFD20_19365) - 4226787..4227296 (+) 510 WP_003102595.1 GspH/FimT family pseudopilin -
  GFD20_RS19370 (GFD20_19370) - 4227402..4227908 (+) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  GFD20_RS19375 (GFD20_19375) pilV 4227899..4228456 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  GFD20_RS19380 (GFD20_19380) pilW 4228453..4229277 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  GFD20_RS19385 (GFD20_19385) pilX 4229274..4229861 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49723.03 Da        Isoelectric Point: 5.8638

>NTDB_id=396979 GFD20_RS19355 WP_003110427.1 4224175..4225512(+) (pilR) [Pseudomonas aeruginosa strain CFSAN084950]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=396979 GFD20_RS19355 WP_003110427.1 4224175..4225512(+) (pilR) [Pseudomonas aeruginosa strain CFSAN084950]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGATATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment