Detailed information NTG0396239

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: GG619_RS12370
• Genome accession: NZ_CP045711
• Coordinates: 2567404..2568018 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain HN-Q-8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2562404..2573018

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GG619_RS12340 (GG619_12340)	rpsT	2562707..2562973 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
GG619_RS12345 (GG619_12345)	holA	2562990..2564031 (-)	1042	Protein_2417	DNA polymerase III subunit delta	-
GG619_RS12350 (GG619_12350)	-	2564071..2564222 (-)	152	Protein_2418	hypothetical protein	-
GG619_RS12355 (GG619_12355)	-	2564263..2564397 (+)	135	WP_003152870.1	YqzM family protein	-
GG619_RS12360 (GG619_12360)	comEC	2564416..2566767 (-)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
GG619_RS12365 (GG619_12365)	-	2566768..2567337 (-)	570	WP_003152868.1	ComE operon protein 2	-
GG619_RS12370 (GG619_12370)	comEA	2567404..2568018 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
GG619_RS12375 (GG619_12375)	comER	2568077..2568898 (+)	822	WP_039254409.1	late competence protein ComER	-
GG619_RS12380 (GG619_12380)	-	2568967..2569704 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
GG619_RS12385 (GG619_12385)	rsfS	2569701..2570057 (-)	357	WP_007408260.1	ribosome silencing factor	-
GG619_RS12390 (GG619_12390)	yqeK	2570075..2570635 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
GG619_RS12395 (GG619_12395)	-	2570625..2571194 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
GG619_RS12400 (GG619_12400)	yhbY	2571205..2571495 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
GG619_RS12405 (GG619_12405)	aroE	2571489..2572331 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=396239 GG619_RS12370 WP_015240245.1 2567404..2568018(-) (comEA) [Bacillus velezensis strain HN-Q-8]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=396239 GG619_RS12370 WP_015240245.1 2567404..2568018(-) (comEA) [Bacillus velezensis strain HN-Q-8]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363