Detailed information NTG0396173

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GHB96_RS08425
• Genome accession: NZ_CP045707
• Coordinates: 1635711..1636571 (+)
• Length: 286 a.a.
• NCBI ID: WP_003689814.1
• Uniprot ID: A0AA44U9R8
• Organism: Neisseria gonorrhoeae strain NG250
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1630711..1641571

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GHB96_RS08410 (GHB96_08895)	dapD	1631221..1632042 (-)	822	WP_003689804.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
GHB96_RS08415 (GHB96_08900)	pgi	1632252..1633895 (-)	1644	WP_003689806.1	glucose-6-phosphate isomerase	-
GHB96_RS08420 (GHB96_08905)	pilG	1634405..1635637 (+)	1233	WP_003689811.1	type 4 pilus assembly protein PilG	Machinery gene
GHB96_RS08425 (GHB96_08910)	pilD	1635711..1636571 (+)	861	WP_003689814.1	prepilin peptidase	Machinery gene
GHB96_RS08430 (GHB96_08915)	coaE	1636568..1637200 (+)	633	WP_003689816.1	dephospho-CoA kinase	-
GHB96_RS08435 (GHB96_08920)	-	1637193..1637402 (+)	210	WP_003696096.1	DNA gyrase inhibitor YacG	-
GHB96_RS08440 (GHB96_08925)	pilF	1637488..1639164 (-)	1677	WP_003689817.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GHB96_RS08450 (GHB96_08940)	-	1639502..1639948 (-)	447	WP_003689818.1	DUF441 domain-containing protein	-
GHB96_RS08455 (GHB96_08945)	-	1639952..1640926 (-)	975	WP_003689819.1	polyprenyl synthetase family protein	-
GHB96_RS08465 (GHB96_08955)	rplU	1641150..1641458 (+)	309	WP_003691838.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31332.34 Da
• Isoelectric Point: 8.9561

>NTDB_id=396173 GHB96_RS08425 WP_003689814.1 1635711..1636571(+) (pilD) [Neisseria gonorrhoeae strain NG250]
MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVR

Nucleotide

• Length: 861 bp

>NTDB_id=396173 GHB96_RS08425 WP_003689814.1 1635711..1636571(+) (pilD) [Neisseria gonorrhoeae strain NG250]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGGCAGCAGTTTTGGGGCTGCTGGTCGGCAGCTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTTATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCGCTGA
CCGACGATGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGTCCCAAATGCCGTGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGTTACCTGCTCCTGCGCGGCAAATGCGCTTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGGTCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATCTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATAGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCTCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCGGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	100	100	1
pilD	Vibrio cholerae strain A1552	44.792	100	0.451
pilD	Vibrio campbellii strain DS40M4	45.652	96.503	0.441
pilD	Acinetobacter baumannii D1279779	47.727	92.308	0.441
pilD	Acinetobacter nosocomialis M2	47.727	92.308	0.441