Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GHB96_RS08440 Genome accession   NZ_CP045707
Coordinates   1637488..1639164 (-) Length   558 a.a.
NCBI ID   WP_003689817.1    Uniprot ID   A0AAQ1E824
Organism   Neisseria gonorrhoeae strain NG250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632488..1644164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHB96_RS08420 (GHB96_08905) pilG 1634405..1635637 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  GHB96_RS08425 (GHB96_08910) pilD 1635711..1636571 (+) 861 WP_003689814.1 prepilin peptidase Machinery gene
  GHB96_RS08430 (GHB96_08915) coaE 1636568..1637200 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  GHB96_RS08435 (GHB96_08920) - 1637193..1637402 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  GHB96_RS08440 (GHB96_08925) pilF 1637488..1639164 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GHB96_RS08450 (GHB96_08940) - 1639502..1639948 (-) 447 WP_003689818.1 DUF441 domain-containing protein -
  GHB96_RS08455 (GHB96_08945) - 1639952..1640926 (-) 975 WP_003689819.1 polyprenyl synthetase family protein -
  GHB96_RS08465 (GHB96_08955) rplU 1641150..1641458 (+) 309 WP_003691838.1 50S ribosomal protein L21 -
  GHB96_RS08470 (GHB96_08960) rpmA 1641483..1641755 (+) 273 WP_003689821.1 50S ribosomal protein L27 -
  GHB96_RS08475 (GHB96_08965) ubiM 1641990..1643174 (+) 1185 WP_010951321.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  GHB96_RS08480 (GHB96_08970) rpmG 1643363..1643518 (-) 156 WP_003689824.1 50S ribosomal protein L33 -
  GHB96_RS08485 (GHB96_08975) rpmB 1643550..1643783 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61863.00 Da        Isoelectric Point: 5.5420

>NTDB_id=396174 GHB96_RS08440 WP_003689817.1 1637488..1639164(-) (pilF) [Neisseria gonorrhoeae strain NG250]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=396174 GHB96_RS08440 WP_003689817.1 1637488..1639164(-) (pilF) [Neisseria gonorrhoeae strain NG250]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACCCAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAAGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGTAGCGG
GGCATCCGACATCCATTTCGAGTTTTACGAACACAATGCGCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGTAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTCCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCTTACGGGATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACACTGCACACGAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGTTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGGTATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAATTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

100

100

1
  pilB Acinetobacter baumannii D1279779

50.088

100

0.509
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.461
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.885

93.907

0.375

Multiple sequence alignment