Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   GFU70_RS24440 Genome accession   NZ_CP045701
Coordinates   5636774..5638363 (+) Length   529 a.a.
NCBI ID   WP_153388919.1    Uniprot ID   A0AAJ3FVQ3
Organism   Pseudomonas brassicacearum strain S-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5631774..5643363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU70_RS24420 (GFU70_24415) pgeF 5633570..5634295 (-) 726 WP_153388918.1 peptidoglycan editing factor PgeF -
  GFU70_RS24425 (GFU70_24420) rluD 5634292..5635254 (-) 963 WP_003205696.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  GFU70_RS24430 (GFU70_24425) - 5635405..5636421 (+) 1017 WP_058546854.1 outer membrane protein assembly factor BamD -
  GFU70_RS24435 (GFU70_24430) - 5636548..5636784 (+) 237 WP_058546855.1 PP0621 family protein -
  GFU70_RS24440 (GFU70_24435) pilS 5636774..5638363 (+) 1590 WP_153388919.1 two-component system sensor histidine kinase NtrB Regulator
  GFU70_RS24445 (GFU70_24440) pilR 5638360..5639706 (+) 1347 WP_058546857.1 sigma-54-dependent transcriptional regulator Regulator
  GFU70_RS24450 (GFU70_24445) - 5639789..5640154 (-) 366 WP_039014157.1 DUF6124 family protein -
  GFU70_RS24455 (GFU70_24450) thiO 5642089..5643189 (-) 1101 WP_153388920.1 glycine oxidase ThiO -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 59047.00 Da        Isoelectric Point: 8.1973

>NTDB_id=396111 GFU70_RS24440 WP_153388919.1 5636774..5638363(+) (pilS) [Pseudomonas brassicacearum strain S-1]
MIAEASSPREKQAQRLLRLYHLYRLSIGITLVLLISSKLDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGVPSAIGNLLIVSVAIGNTLLRSRVGLLIAAVATIGIVGSTFFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAERRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLVGQRID
DYCTALVERLQLWLNNPSLRPPSLTVTGTGLTLQPSFIALGHYDQQQFLVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNNADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRAWLEQFVRQARESMAEH
QHLHLSIDPGQYTTLMDPDQLTQVLDNLLRNAWRHSAMAHEQAEAWLKLFIDPHSQLSTLDIIDNGPGVTPERQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=396111 GFU70_RS24440 WP_153388919.1 5636774..5638363(+) (pilS) [Pseudomonas brassicacearum strain S-1]
GTGATCGCTGAGGCCTCGAGCCCACGCGAAAAACAGGCGCAGCGCCTGCTGCGCCTCTATCACCTGTATCGCCTGAGCAT
CGGCATCACCCTGGTGCTACTGATATCCAGCAAGCTGGACAACCGCCTGTTGGAGTTCGCCAATGACGACCTGCTGCGCA
GTGGCAGTTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTCCTTGAGAATACTCGCCGCCCCGCCCGGCTGTTC
GGCCTGGCACTCACCGATGTGCTGCTGCTCTCGTGGCTGTTCTTCGCCGCAGGTGGTGTGCCCAGCGCCATCGGCAATCT
GCTCATTGTCTCGGTAGCCATCGGCAATACGCTGTTGCGAAGCCGGGTCGGCCTATTGATCGCCGCTGTCGCCACGATCG
GTATCGTCGGGTCGACTTTTTTCCTCGGCCTGAGCGACTCGAACCGTCCCAGCAGTTATCTGCAAGCCGGCACCCTGGGG
GCACTGTGTTTTGCCGCCGCCCTGCTGGTACAAGGCCTGACCCGGCGACTGGAAGCCAGTGAAACCCTGGCCGAGCGACG
GGCCAGCGAGGTGATCGGCCTCGAAGCGCTCAACGCACTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTCGACC
GCGAGCGGCGCGTGCAACTGGCCAACGAAAGCGCCCTGAACCTGCTGGGCATGCACGACCTGGTTGGCCAGCGGATCGAT
GATTATTGCACCGCCCTGGTCGAACGCCTGCAATTGTGGCTGAACAACCCCAGCCTGCGTCCCCCCAGCCTGACCGTGAC
CGGCACAGGCCTGACCCTGCAACCGAGCTTCATCGCCTTGGGGCACTACGATCAGCAACAGTTCCTGGTCTTCCTTGAAG
ACCTGGCCCAGGTTGCCCAACAGGCCCAGCAACTGAAACTCGCCTCCCTCGGGCGCCTCACCGCTGGCATTGCCCATGAA
ATCCGCAACCCTTTGGGCGCCATCAGCCATGCAGCGCAATTACTGCGCGAATCCGAGGAACTGAACAACGCTGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAACGAATGAATCGCGTCATTGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AGACCACGCCGCAACGCCTGGACCTGCGGGCCTGGCTCGAACAGTTCGTCCGTCAGGCCCGCGAAAGCATGGCCGAGCAC
CAACACTTGCACCTGAGCATCGACCCGGGGCAATACACCACGCTGATGGACCCCGACCAGTTGACCCAGGTACTCGACAA
CCTGCTGCGCAACGCCTGGCGCCACAGCGCCATGGCCCACGAACAGGCCGAGGCCTGGCTGAAACTGTTCATCGACCCCC
ACAGCCAGCTGTCCACGCTGGACATCATCGACAACGGCCCCGGTGTAACACCCGAGCGGCAGGCGCATTTGTTCGAACCC
TTCTTTACCACCAGCAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGTTGCTTTCGCATTACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.038

99.811

0.599

Multiple sequence alignment