Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HPM96_RS21180 Genome accession   NZ_CP053852
Coordinates   4390596..4391360 (+) Length   254 a.a.
NCBI ID   WP_001136211.1    Uniprot ID   -
Organism   Escherichia coli strain CE1628     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4385596..4396360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPM96_RS21160 (HPM96_21155) acpT 4386550..4387137 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  HPM96_RS21165 (HPM96_21160) nikA 4387248..4388822 (+) 1575 WP_000493131.1 nickel ABC transporter substrate-binding protein -
  HPM96_RS21170 (HPM96_21165) nikB 4388822..4389766 (+) 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
  HPM96_RS21175 (HPM96_21170) nikC 4389763..4390596 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  HPM96_RS21180 (HPM96_21175) amiE 4390596..4391360 (+) 765 WP_001136211.1 nickel import ATP-binding protein NikD Regulator
  HPM96_RS21185 (HPM96_21180) nikE 4391357..4392163 (+) 807 WP_061092823.1 nickel import ATP-binding protein NikE -
  HPM96_RS21190 (HPM96_21185) nikR 4392168..4392569 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HPM96_RS21195 (HPM96_21190) - 4392689..4393048 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  HPM96_RS21200 (HPM96_21195) - 4393045..4393320 (-) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  HPM96_RS21205 (HPM96_21200) yhhJ 4393393..4394517 (-) 1125 WP_001314210.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.45 Da        Isoelectric Point: 6.9016

>NTDB_id=395907 HPM96_RS21180 WP_001136211.1 4390596..4391360(+) (amiE) [Escherichia coli strain CE1628]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=395907 HPM96_RS21180 WP_001136211.1 4390596..4391360(+) (amiE) [Escherichia coli strain CE1628]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCGGATGACGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment