Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HPY17_RS11725 Genome accession   NZ_CP053816
Coordinates   2615196..2616923 (-) Length   575 a.a.
NCBI ID   WP_001122161.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA7G     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2610196..2621923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY17_RS11690 (HPY17_11695) - 2610499..2611269 (-) 771 WP_175247561.1 PilW family protein -
  HPY17_RS11695 (HPY17_11700) - 2611269..2611838 (-) 570 WP_175247562.1 type II secretion system protein -
  HPY17_RS11700 (HPY17_11705) - 2611828..2612340 (-) 513 WP_175247563.1 type II secretion system protein -
  HPY17_RS11705 (HPY17_11710) - 2612402..2612839 (-) 438 WP_142731686.1 type II secretion system protein -
  HPY17_RS11710 (HPY17_11715) - 2612874..2613428 (-) 555 WP_000738707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY17_RS11715 (HPY17_11720) - 2613509..2613961 (-) 453 WP_000689644.1 hypothetical protein -
  HPY17_RS11720 (HPY17_11725) - 2613964..2615187 (-) 1224 WP_175247564.1 type II secretion system F family protein -
  HPY17_RS11725 (HPY17_11730) pilB/pilB1 2615196..2616923 (-) 1728 WP_001122161.1 GspE/PulE family protein Machinery gene
  HPY17_RS11730 (HPY17_11735) - 2616913..2618064 (-) 1152 WP_175247565.1 lipopolysaccharide assembly protein LapB -
  HPY17_RS11735 (HPY17_11740) - 2618061..2618906 (-) 846 WP_000281360.1 ExeA family protein -
  HPY17_RS11740 (HPY17_11745) mshL 2618911..2620590 (-) 1680 WP_001227123.1 pilus (MSHA type) biogenesis protein MshL -
  HPY17_RS11745 (HPY17_11750) - 2620605..2620922 (-) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  HPY17_RS11750 (HPY17_11755) gspM 2620915..2621565 (-) 651 WP_000835144.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63858.27 Da        Isoelectric Point: 5.9153

>NTDB_id=395583 HPY17_RS11725 WP_001122161.1 2615196..2616923(-) (pilB/pilB1) [Vibrio cholerae strain SA7G]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRANVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNHLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAQFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=395583 HPY17_RS11725 WP_001122161.1 2615196..2616923(-) (pilB/pilB1) [Vibrio cholerae strain SA7G]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTCAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTAATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGGGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCACTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AAAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTCTCGTTTGCCGAGCAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTACTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCAGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCGGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGTGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGAATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCTGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCGGTCGATAGCGCGCT
GCGTTTGATTGATATGGGTGCGCCCGGTTATCTGGTCGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTGCGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCCAATTTACTCGTGGACGCGGCTGCCAAAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTCGA
AATGCTTGAGCTGGATCAGCCTATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCAGCGATGGAGTTAGCGCTGCAAGGTATCGTCAGTCTTGATGAAGTGATGTCG
CTCGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATTTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.62

98.783

0.362
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362
  pilB Legionella pneumophila strain ERS1305867

39.057

92.174

0.36

Multiple sequence alignment