Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HPY06_RS11630 Genome accession   NZ_CP053804
Coordinates   2576025..2577752 (-) Length   575 a.a.
NCBI ID   WP_001122155.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL6Y     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2571025..2582752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY06_RS11595 (HPY06_11600) - 2571286..2572056 (-) 771 WP_053034679.1 PilW family protein -
  HPY06_RS11600 (HPY06_11605) - 2572056..2572625 (-) 570 WP_053034680.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY06_RS11605 (HPY06_11610) - 2572615..2573127 (-) 513 WP_001249944.1 type II secretion system protein -
  HPY06_RS11610 (HPY06_11615) - 2573192..2573668 (-) 477 WP_002051008.1 type II secretion system protein -
  HPY06_RS11615 (HPY06_11620) - 2573703..2574257 (-) 555 WP_000738708.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY06_RS11620 (HPY06_11625) - 2574338..2574790 (-) 453 WP_000689643.1 hypothetical protein -
  HPY06_RS11625 (HPY06_11630) - 2574793..2576016 (-) 1224 WP_053034681.1 type II secretion system F family protein -
  HPY06_RS11630 (HPY06_11635) pilB/pilB1 2576025..2577752 (-) 1728 WP_001122155.1 GspE/PulE family protein Machinery gene
  HPY06_RS11635 (HPY06_11640) - 2577742..2578893 (-) 1152 WP_175230826.1 lipopolysaccharide assembly protein LapB -
  HPY06_RS11640 (HPY06_11645) - 2578890..2579735 (-) 846 WP_057550605.1 ExeA family protein -
  HPY06_RS11645 (HPY06_11650) mshL 2579740..2581404 (-) 1665 WP_142548734.1 pilus (MSHA type) biogenesis protein MshL -
  HPY06_RS11650 (HPY06_11655) - 2581419..2581736 (-) 318 WP_001887862.1 MSHA biogenesis protein MshK -
  HPY06_RS11655 (HPY06_11660) gspM 2581729..2582379 (-) 651 WP_000835144.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63872.34 Da        Isoelectric Point: 6.0455

>NTDB_id=395166 HPY06_RS11630 WP_001122155.1 2576025..2577752(-) (pilB/pilB1) [Vibrio cholerae strain SL6Y]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=395166 HPY06_RS11630 WP_001122155.1 2576025..2577752(-) (pilB/pilB1) [Vibrio cholerae strain SL6Y]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTCATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGGGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTATGGTTTTGAGTTTGTCATCGCCCCTG
AGAAGCAGTTGGTCGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAAATTGTTTCGTTCGCCGAACAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGTC
AACGGATCGATGGGGTATTGCATGAAACCTTGCTCCATGAAGTGAATATCGCCTCAGCCTTAGTGCTGCGCTTAAAGTTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGACGCTTTAACATCCGTGTAAAAGGACAATCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCGGTGGTGATGCGTTTGCTCAACCAATCTGCAGGGGTTAGAA
AGCTCGAAGAGTCGGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGTGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGAATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCCGCCTTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGCGCGCCCGGTTATCTGGTCGCCAGTGCGGTACGTGCTGTGGTTGCGCAGCGTTTAGTGCGAA
AAGTCTGTCCAGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGCCAAAACTGTAACTTAACCGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTGCAAGGCATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTGAACCGATTTATTTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362
  pilB Legionella pneumophila strain ERS1305867

39.057

92.174

0.36