Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   GCU76_RS13905 Genome accession   NZ_CP045425
Coordinates   2559426..2560043 (-) Length   205 a.a.
NCBI ID   WP_017696282.1    Uniprot ID   -
Organism   Bacillus subtilis strain JAAA     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2554426..2565043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU76_RS13875 rpsT 2554728..2554994 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  GCU76_RS13880 holA 2555009..2556052 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  GCU76_RS13885 - 2556092..2556241 (-) 150 WP_003229985.1 hypothetical protein -
  GCU76_RS13890 yqzM 2556282..2556416 (+) 135 WP_003229983.1 YqzM family protein -
  GCU76_RS13895 comEC 2556456..2558786 (-) 2331 WP_017696281.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GCU76_RS13900 comEB 2558790..2559359 (-) 570 WP_003229978.1 ComE operon protein 2 -
  GCU76_RS13905 comEA 2559426..2560043 (-) 618 WP_017696282.1 competence protein ComEA Machinery gene
  GCU76_RS13910 comER 2560127..2560948 (+) 822 WP_004398597.1 late competence protein ComER -
  GCU76_RS13915 yqeM 2561014..2561757 (-) 744 WP_017696283.1 class I SAM-dependent methyltransferase -
  GCU76_RS13920 rsfS 2561754..2562110 (-) 357 WP_014480315.1 ribosome silencing factor -
  GCU76_RS13925 yqeK 2562128..2562688 (-) 561 WP_017696284.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  GCU76_RS13930 nadD 2562678..2563247 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  GCU76_RS13935 yhbY 2563259..2563549 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  GCU76_RS13940 aroE 2563543..2564385 (-) 843 WP_017696285.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21763.49 Da        Isoelectric Point: 4.7220

>NTDB_id=394000 GCU76_RS13905 WP_017696282.1 2559426..2560043(-) (comEA) [Bacillus subtilis strain JAAA]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEGNNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=394000 GCU76_RS13905 WP_017696282.1 2559426..2560043(-) (comEA) [Bacillus subtilis strain JAAA]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGAAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGGAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGACCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.818

100

0.395

Multiple sequence alignment