Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HPK14_RS03600 Genome accession   NZ_CP053662
Coordinates   739205..739864 (-) Length   219 a.a.
NCBI ID   WP_001221510.1    Uniprot ID   -
Organism   Escherichia coli strain eco-241     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 734205..744864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPK14_RS03575 (HPK14_03530) parE 734296..736188 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  HPK14_RS03580 (HPK14_03535) ygiN 736236..736550 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  HPK14_RS03585 (HPK14_03540) mdaB 736581..737162 (-) 582 WP_000072421.1 NADPH:quinone oxidoreductase MdaB -
  HPK14_RS03590 (HPK14_03545) ygiZ 737481..737813 (+) 333 WP_000917684.1 DUF2645 family protein -
  HPK14_RS03595 (HPK14_03550) qseC 737859..739208 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  HPK14_RS03600 (HPK14_03555) ciaR 739205..739864 (-) 660 WP_001221510.1 quorum sensing response regulator transcription factor QseB Regulator
  HPK14_RS03605 (HPK14_03560) ygiW 740016..740408 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HPK14_RS03610 (HPK14_03565) ygiV 740461..740943 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  HPK14_RS03615 (HPK14_03570) mqsR 741148..741444 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  HPK14_RS03620 (HPK14_03575) mqsA 741446..741841 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  HPK14_RS03625 (HPK14_03580) ygiS 741974..743581 (+) 1608 WP_001735743.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24686.69 Da        Isoelectric Point: 8.0731

>NTDB_id=393244 HPK14_RS03600 WP_001221510.1 739205..739864(-) (ciaR) [Escherichia coli strain eco-241]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILRKWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=393244 HPK14_RS03600 WP_001221510.1 739205..739864(-) (ciaR) [Escherichia coli strain eco-241]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCAAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361