Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GA636_RS01465 Genome accession   NZ_CP045135
Coordinates   301261..302121 (+) Length   286 a.a.
NCBI ID   WP_005181774.1    Uniprot ID   -
Organism   Acinetobacter indicus strain XG01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 296261..307121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA636_RS01445 secG 296890..297219 (-) 330 WP_016659654.1 preprotein translocase subunit SecG -
  GA636_RS01450 tpiA 297233..298024 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  GA636_RS01455 pilB 298313..300019 (+) 1707 WP_075167609.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GA636_RS01460 pilC 300050..301261 (+) 1212 WP_160232118.1 type II secretion system F family protein Machinery gene
  GA636_RS01465 pilD 301261..302121 (+) 861 WP_005181774.1 prepilin peptidase Machinery gene
  GA636_RS01470 coaE 302132..302740 (+) 609 WP_075175011.1 dephospho-CoA kinase -
  GA636_RS01475 - 302737..303657 (-) 921 WP_016659657.1 DMT family transporter -
  GA636_RS01480 rlmB 303675..304427 (-) 753 WP_075167607.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GA636_RS01485 - 304533..304856 (-) 324 WP_075167606.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32048.33 Da        Isoelectric Point: 7.8299

>NTDB_id=392204 GA636_RS01465 WP_005181774.1 301261..302121(+) (pilD) [Acinetobacter indicus strain XG01]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKISLSQPASTCPKCHSP
IRWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=392204 GA636_RS01465 WP_005181774.1 301261..302121(+) (pilD) [Acinetobacter indicus strain XG01]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCATCATTGATGAAAGCAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCGCTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAATGCCAAAACCCGATCAG
TATTCGTTATCCTTTTATTGAACTGCTGACCGCAGTGTGTGCCTTAACCGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTGCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCTAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCTTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Acinetobacter baumannii D1279779

79.649

99.65

0.794
  pilD Vibrio cholerae strain A1552

55.431

93.357

0.517
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437

Multiple sequence alignment