NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	HHJ51_RS14755	Genome accession	NZ_CP053247
Coordinates	3027310..3027969 (-)	Length	219 a.a.
NCBI ID	WP_001221493.1	Uniprot ID	Q3YXL4
Organism	Escherichia coli strain SCU-482
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3022310..3032969

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HHJ51_RS14730 (HHJ51_14725)	ygiN	3023200..3023514 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
HHJ51_RS14735 (HHJ51_14730)	mdaB	3023545..3024126 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
HHJ51_RS26270	-	3024375..3024853 (+)	479	Protein_2916	Hcp family type VI secretion system effector	-
HHJ51_RS26275	-	3024858..3025566 (+)	709	Protein_2917	hypothetical protein	-
HHJ51_RS14745 (HHJ51_14740)	-	3025630..3025918 (+)	289	Protein_2918	DUF2645 family protein	-
HHJ51_RS14750 (HHJ51_14745)	qseC	3025964..3027313 (-)	1350	WP_000673357.1	quorum sensing histidine kinase QseC	-
HHJ51_RS14755 (HHJ51_14750)	ciaR	3027310..3027969 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
HHJ51_RS14760 (HHJ51_14755)	ygiW	3028121..3028513 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HHJ51_RS14765 (HHJ51_14760)	ygiV	3028566..3029048 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
HHJ51_RS14770 (HHJ51_14765)	mqsR	3029253..3029549 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
HHJ51_RS14775 (HHJ51_14770)	mqsA	3029551..3029946 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
HHJ51_RS14780 (HHJ51_14775)	ygiS	3030079..3031686 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24687.63 Da Isoelectric Point: 6.9850

                                >NTDB_id=391013 HHJ51_RS14755 WP_001221493.1 3027310..3027969(-) (ciaR) [Escherichia coli strain SCU-482]

MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA

RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL

LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

Download Length: 660 bp

                                >NTDB_id=391013 HHJ51_RS14755 WP_001221493.1 3027310..3027969(-) (ciaR) [Escherichia coli strain SCU-482]

ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT

CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT

TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAGAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG

CGTGATGCGTTAGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA

AGTCGCCGCCAGGCTGGAGGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC

TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA

CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG

TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT

ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365

Detailed information NTG0391013