Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   F8M43_RS18510 Genome accession   NZ_CP044498
Coordinates   3538763..3540154 (-) Length   463 a.a.
NCBI ID   WP_038427948.1    Uniprot ID   -
Organism   Bacillus subtilis strain ms-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3533763..3545154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS18475 flgL 3533934..3534830 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  F8M43_RS18480 flgK 3534841..3536364 (-) 1524 WP_015714798.1 flagellar hook-associated protein FlgK -
  F8M43_RS18485 flgN 3536383..3536865 (-) 483 WP_003227999.1 flagellar protein FlgN -
  F8M43_RS18490 flgM 3536881..3537147 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  F8M43_RS18495 yvyF 3537228..3537647 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  F8M43_RS18500 comFC 3537721..3538443 (-) 723 WP_038427947.1 comF operon protein ComFC Machinery gene
  F8M43_RS18505 comFB 3538407..3538703 (-) 297 WP_015483764.1 late competence protein ComFB -
  F8M43_RS18510 comFA 3538763..3540154 (-) 1392 WP_038427948.1 ATP-dependent helicase ComFA Machinery gene
  F8M43_RS18515 fakBA 3540260..3541105 (-) 846 WP_003244125.1 DegV family protein -
  F8M43_RS18520 degU 3541203..3541892 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  F8M43_RS18525 degS 3541975..3543132 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  F8M43_RS18530 yvyE 3543349..3544002 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52577.64 Da        Isoelectric Point: 10.0393

>NTDB_id=390590 F8M43_RS18510 WP_038427948.1 3538763..3540154(-) (comFA) [Bacillus subtilis strain ms-2]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=390590 F8M43_RS18510 WP_038427948.1 3538763..3540154(-) (comFA) [Bacillus subtilis strain ms-2]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCGCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCATTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

99.568

100

0.996

Multiple sequence alignment