Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   F8M43_RS13115 Genome accession   NZ_CP044498
Coordinates   2522553..2523170 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain ms-2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2517553..2528170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS13085 rpsT 2517855..2518121 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  F8M43_RS13090 holA 2518136..2519179 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  F8M43_RS13095 - 2519219..2519368 (-) 150 WP_003229985.1 hypothetical protein -
  F8M43_RS13100 yqzM 2519409..2519543 (+) 135 WP_003229983.1 YqzM family protein -
  F8M43_RS13105 comEC 2519583..2521913 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  F8M43_RS13110 comEB 2521917..2522486 (-) 570 WP_003229978.1 ComE operon protein 2 -
  F8M43_RS13115 comEA 2522553..2523170 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  F8M43_RS13120 comER 2523254..2524075 (+) 822 WP_004398597.1 late competence protein ComER -
  F8M43_RS13125 yqeM 2524141..2524884 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  F8M43_RS13130 rsfS 2524881..2525237 (-) 357 WP_003229971.1 ribosome silencing factor -
  F8M43_RS13135 yqeK 2525255..2525815 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  F8M43_RS13140 nadD 2525805..2526374 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  F8M43_RS13145 yhbY 2526386..2526676 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  F8M43_RS13150 aroE 2526670..2527512 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=390574 F8M43_RS13115 WP_004398514.1 2522553..2523170(-) (comEA) [Bacillus subtilis strain ms-2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=390574 F8M43_RS13115 WP_004398514.1 2522553..2523170(-) (comEA) [Bacillus subtilis strain ms-2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment