Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FSC09_RS13385 Genome accession   NZ_CP044455
Coordinates   2748586..2749446 (+) Length   286 a.a.
NCBI ID   WP_005181774.1    Uniprot ID   -
Organism   Acinetobacter indicus strain B18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2743586..2754446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC09_RS13365 (FSC09_13425) secG 2744200..2744529 (-) 330 WP_163146191.1 preprotein translocase subunit SecG -
  FSC09_RS13370 (FSC09_13430) tpiA 2744543..2745334 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  FSC09_RS13375 (FSC09_13435) pilB 2745623..2747329 (+) 1707 WP_163146192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FSC09_RS13380 (FSC09_13440) pilC 2747360..2748586 (+) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  FSC09_RS13385 (FSC09_13445) pilD 2748586..2749446 (+) 861 WP_005181774.1 prepilin peptidase Machinery gene
  FSC09_RS13390 (FSC09_13450) coaE 2749457..2750065 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  FSC09_RS13395 (FSC09_13455) - 2750062..2750982 (-) 921 WP_016659657.1 DMT family transporter -
  FSC09_RS13400 (FSC09_13460) rlmB 2751000..2751752 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FSC09_RS13405 (FSC09_13465) - 2751858..2752181 (-) 324 WP_163146193.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32048.33 Da        Isoelectric Point: 7.8299

>NTDB_id=390137 FSC09_RS13385 WP_005181774.1 2748586..2749446(+) (pilD) [Acinetobacter indicus strain B18]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKISLSQPASTCPKCHSP
IRWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=390137 FSC09_RS13385 WP_005181774.1 2748586..2749446(+) (pilD) [Acinetobacter indicus strain B18]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCATCATTGATGAAAGCAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCGCTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAATGCCAAAACCCGATCAG
TATTCGTTATCCTTTTATTGAACTGCTGACCGCAGTGTGTGCCTTAACCGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTGCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCCAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCTTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Acinetobacter baumannii D1279779

79.649

99.65

0.794
  pilD Vibrio cholerae strain A1552

55.431

93.357

0.517
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437

Multiple sequence alignment