Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   F7V86_RS19960 Genome accession   NZ_CP044444
Coordinates   3953995..3954609 (-) Length   204 a.a.
NCBI ID   WP_017418180.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain KC41     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3948995..3959609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7V86_RS19930 rpsT 3949298..3949564 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  F7V86_RS19935 holA 3949581..3950622 (-) 1042 Protein_3851 DNA polymerase III subunit delta -
  F7V86_RS19940 - 3950662..3950813 (-) 152 Protein_3852 hypothetical protein -
  F7V86_RS19945 - 3950854..3950988 (+) 135 WP_003152870.1 YqzM family protein -
  F7V86_RS19950 comEC 3951007..3953358 (-) 2352 WP_151140321.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  F7V86_RS19955 - 3953359..3953928 (-) 570 WP_003152868.1 ComE operon protein 2 -
  F7V86_RS19960 comEA 3953995..3954609 (-) 615 WP_017418180.1 helix-hairpin-helix domain-containing protein Machinery gene
  F7V86_RS19965 comER 3954668..3955489 (+) 822 WP_014305449.1 late competence protein ComER -
  F7V86_RS19970 - 3955558..3956295 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  F7V86_RS19975 rsfS 3956292..3956648 (-) 357 WP_003152864.1 ribosome silencing factor -
  F7V86_RS19980 yqeK 3956645..3957226 (-) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  F7V86_RS19985 - 3957216..3957785 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  F7V86_RS19990 yhbY 3957796..3958086 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  F7V86_RS19995 aroE 3958080..3958922 (-) 843 WP_017418183.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22218.33 Da        Isoelectric Point: 5.4841

>NTDB_id=390020 F7V86_RS19960 WP_017418180.1 3953995..3954609(-) (comEA) [Bacillus amyloliquefaciens strain KC41]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=390020 F7V86_RS19960 WP_017418180.1 3953995..3954609(-) (comEA) [Bacillus amyloliquefaciens strain KC41]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGTGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment