Detailed information NTG0038937

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NMBH4476_RS00265
• Genome accession: NC_017516
• Coordinates: 55400..56626 (-)
• Length: 408 a.a.
• NCBI ID: WP_002218483.1
• Uniprot ID: Q9K1N4
• Organism: Neisseria meningitidis H44/76
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 50400..61626

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMBH4476_RS00260 (NMBH4476_0047)	yccS	52964..55114 (-)	2151	WP_002221798.1	YccS family putative transporter	-
NMBH4476_RS00265 (NMBH4476_0048)	pilU	55400..56626 (-)	1227	WP_002218483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NMBH4476_RS00270 (NMBH4476_0049)	pilT	56789..57832 (-)	1044	WP_002218487.1	type IV pilus twitching motility protein PilT	Machinery gene
NMBH4476_RS00275 (NMBH4476_0050)	-	57952..58638 (+)	687	WP_002243931.1	YggS family pyridoxal phosphate-dependent enzyme	-
NMBH4476_RS00280 (NMBH4476_0051)	-	58638..59045 (+)	408	WP_002243932.1	hypothetical protein	-
NMBH4476_RS00285 (NMBH4476_0052)	proC	59076..59885 (+)	810	WP_225905354.1	pyrroline-5-carboxylate reductase	-
NMBH4476_RS00290 (NMBH4476_0053)	dksA	60032..60448 (+)	417	WP_002215267.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45674.28 Da
• Isoelectric Point: 6.9640

>NTDB_id=38937 NMBH4476_RS00265 WP_002218483.1 55400..56626(-) (pilU) [Neisseria meningitidis H44/76]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=38937 NMBH4476_RS00265 WP_002218483.1 55400..56626(-) (pilU) [Neisseria meningitidis H44/76]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAATTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q9K1N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.663	87.255	0.39
pilU	Vibrio cholerae strain A1552	46.429	82.353	0.382