Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   F3129_RS12240 Genome accession   NZ_CP044133
Coordinates   2525633..2526703 (-) Length   356 a.a.
NCBI ID   WP_150941313.1    Uniprot ID   -
Organism   Bacillus velezensis strain FJAT-46737     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2520633..2531703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F3129_RS12190 (F3129_12190) sipW 2520693..2521277 (-) 585 WP_015240205.1 signal peptidase I SipW -
  F3129_RS12195 (F3129_12195) tapA 2521249..2521920 (-) 672 WP_150941310.1 amyloid fiber anchoring/assembly protein TapA -
  F3129_RS12200 (F3129_12200) - 2522179..2522508 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  F3129_RS12205 (F3129_12205) - 2522548..2522727 (-) 180 WP_003153093.1 YqzE family protein -
  F3129_RS12210 (F3129_12210) comGG 2522784..2523161 (-) 378 WP_150941311.1 competence type IV pilus minor pilin ComGG Machinery gene
  F3129_RS12215 (F3129_12215) comGF 2523162..2523662 (-) 501 WP_256994853.1 competence type IV pilus minor pilin ComGF -
  F3129_RS12220 (F3129_12220) comGE 2523571..2523885 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  F3129_RS12225 (F3129_12225) comGD 2523869..2524306 (-) 438 WP_015240210.1 competence type IV pilus minor pilin ComGD Machinery gene
  F3129_RS12230 (F3129_12230) comGC 2524296..2524604 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  F3129_RS12235 (F3129_12235) comGB 2524609..2525646 (-) 1038 WP_150941312.1 competence type IV pilus assembly protein ComGB Machinery gene
  F3129_RS12240 (F3129_12240) comGA 2525633..2526703 (-) 1071 WP_150941313.1 competence type IV pilus ATPase ComGA Machinery gene
  F3129_RS12245 (F3129_12245) - 2526896..2527846 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  F3129_RS12250 (F3129_12250) - 2527992..2529293 (+) 1302 WP_012117986.1 hemolysin family protein -
  F3129_RS12255 (F3129_12255) - 2529331..2530161 (-) 831 WP_015240214.1 STAS domain-containing protein -
  F3129_RS12265 (F3129_12265) - 2530368..2530748 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  F3129_RS12270 (F3129_12270) - 2530985..2531230 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40502.74 Da        Isoelectric Point: 9.0316

>NTDB_id=388180 F3129_RS12240 WP_150941313.1 2525633..2526703(-) (comGA) [Bacillus velezensis strain FJAT-46737]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTQRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=388180 F3129_RS12240 WP_150941313.1 2525633..2526703(-) (comGA) [Bacillus velezensis strain FJAT-46737]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACAACGTGCAGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379

Multiple sequence alignment