Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HHJ43_RS03545 Genome accession   NZ_CP051751
Coordinates   749741..750400 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain SCU-301     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 744741..755400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ43_RS03515 (HHJ43_03520) - 744857..745804 (+) 948 WP_001305106.1 iron-siderophore ABC transporter substrate-binding protein -
  HHJ43_RS03520 (HHJ43_03525) - 745801..746688 (+) 888 WP_033560346.1 MurR/RpiR family transcriptional regulator -
  HHJ43_RS03525 (HHJ43_03530) ygiN 746733..747047 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  HHJ43_RS03530 (HHJ43_03535) mdaB 747078..747659 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HHJ43_RS03535 (HHJ43_03540) ygiZ 748017..748349 (+) 333 WP_022646136.1 DUF2645 family protein -
  HHJ43_RS03540 (HHJ43_03545) qseC 748395..749744 (-) 1350 WP_000673371.1 quorum sensing histidine kinase QseC -
  HHJ43_RS03545 (HHJ43_03550) ciaR 749741..750400 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  HHJ43_RS03550 (HHJ43_03555) ygiW 750552..750944 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HHJ43_RS03555 (HHJ43_03560) ygiV 750997..751479 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  HHJ43_RS03560 (HHJ43_03565) ygiS 751588..753195 (+) 1608 WP_096987017.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=387901 HHJ43_RS03545 WP_001221493.1 749741..750400(-) (ciaR) [Escherichia coli strain SCU-301]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=387901 HHJ43_RS03545 WP_001221493.1 749741..750400(-) (ciaR) [Escherichia coli strain SCU-301]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTTTTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAGCCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365