Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   FOB77_RS02220 Genome accession   NZ_CP044091
Coordinates   402100..403389 (-) Length   429 a.a.
NCBI ID   WP_000432954.1    Uniprot ID   A0AAW6XXN4
Organism   Streptococcus agalactiae strain FDAARGOS_669     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 397100..408389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB77_RS02210 (FOB77_02210) hpf 400804..401358 (-) 555 WP_000599096.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  FOB77_RS02215 (FOB77_02215) - 401435..402100 (-) 666 WP_150371496.1 ComF family protein -
  FOB77_RS02220 (FOB77_02220) comFA/cflA 402100..403389 (-) 1290 WP_000432954.1 DEAD/DEAH box helicase Machinery gene
  FOB77_RS02225 (FOB77_02225) - 403445..404089 (+) 645 WP_001108144.1 YigZ family protein -
  FOB77_RS02230 (FOB77_02230) cysK 404180..405106 (+) 927 WP_000036944.1 cysteine synthase A -
  FOB77_RS02235 (FOB77_02235) - 405258..406346 (-) 1089 WP_000862123.1 glycerol dehydrogenase -
  FOB77_RS02240 (FOB77_02240) - 406414..407082 (-) 669 WP_000394891.1 fructose-6-phosphate aldolase -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 48730.56 Da        Isoelectric Point: 9.9941

>NTDB_id=387662 FOB77_RS02220 WP_000432954.1 402100..403389(-) (comFA/cflA) [Streptococcus agalactiae strain FDAARGOS_669]
MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSVAKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTF
SKTNSLKWKGGLTPYQNEVSEELLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHGVKQALKENGTSIFLTATSTTEL
ERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQKSLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEMLRQ
AFPMEKIGFVSSKSTSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVGRALERPEG
LLYFLHDGKSKSMHQAIKEIKNMNHIGGF

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=387662 FOB77_RS02220 WP_000432954.1 402100..403389(-) (comFA/cflA) [Streptococcus agalactiae strain FDAARGOS_669]
ATGGAAAATTATTTAGGTAGACTTTGGACAAAAGCACAATTATCTGAGCAACTTAGAAAAATTGCAATTAACTTGCCATC
TTTTATAAAGAAAGGAAGCGACTATATTTGTACTCGATGCTCTAGTTCTGTTGCCAAAAATTGCCAATTACCAACTGGAA
ATTATTATTGTAGAGAATGTATAGTTTTTGGAAGAGTTACTAGTAATGAAAATTTATATTATTTCCCACAAAAAACATTT
TCTAAAACGAATTCTTTAAAATGGAAAGGGGGACTGACACCTTATCAAAATGAGGTTTCAGAAGAGTTGTTGAAAGGTAT
TTCAAGTAAGGAAAATCTTCTTGTTCATGCTGTGACAGGGGCTGGTAAAACAGAAATGATATACCACTCTATCGCCAAAG
TAATTGATACTGGAGGAAGCGTTTGTATTGCCAGTCCTAGAATTGATGTTTGTTTAGAATTGCATAAGCGACTTTCAAAC
GATTTTAGATGTGCCATAACATTAATGCATGGAGAATCTCCTTCTTATCAGAGATCCCCTTTAACTATTGCCACAACACA
TCAATTGTTAAAATTTTATCATGCTTTTGATTTGCTAATTGTTGATGAAGTTGATGCTTTTCCATATGTCGACAACCCGA
TACTATACCATGGTGTAAAACAGGCCTTAAAGGAAAACGGAACAAGTATTTTTCTGACTGCAACTTCAACGACTGAGTTA
GAAAGAAAAGTTGCAAGAAAAGAACTAAAAAAATTACATCTAGCTAGGAGATTTCATGCCAATCCGTTAGTGATACCCGA
GATGGTTTGGGTAAGTGGTATCCAGAAAAGCTTGCAGACACAAAAACTACCTCCCAAATTATATCAGCTGATAAATAAAC
AGCGACAAACGAGATATCCTTTGTTATTATTTTTCCCCCATATTTCAGAAGGTCAGGTATTCACCGAAATGTTAAGACAA
GCCTTTCCTATGGAAAAAATTGGTTTTGTTTCAAGTAAGAGCACATCTAGGTTAAAGTTAGTGCAAGATTTTCGAGATAA
TAAATTGTCTATCCTAGTGTCAACTACCATTCTAGAGAGAGGAGTAACTTTCCCTAGCGTCGACGTTTTTGTTATACAAG
CAAATCATCATTTGTTTACGAAAAGTTCCTTGGTACAAATTTCAGGAAGAGTTGGAAGAGCATTAGAAAGGCCCGAAGGG
CTCCTTTACTTCCTTCATGATGGGAAAAGTAAATCGATGCACCAAGCTATAAAGGAGATTAAAAACATGAATCATATAGG
AGGATTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(286-394)

DEAD/DEAH box helicase (DEAD)

(93-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae Rx1

59.953

99.534

0.597
  comFA/cflA Streptococcus pneumoniae D39

59.953

99.534

0.597
  comFA/cflA Streptococcus pneumoniae R6

59.953

99.534

0.597
  comFA/cflA Streptococcus pneumoniae TIGR4

59.953

99.534

0.597
  comFA/cflA Streptococcus mitis SK321

59.953

99.534

0.597
  comFA Lactococcus lactis subsp. cremoris KW2

50.254

91.841

0.462
  comFA Latilactobacillus sakei subsp. sakei 23K

37.037

100

0.373
  comFA Bacillus subtilis subsp. subtilis str. 168

39.312

94.872

0.373

Multiple sequence alignment