Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   FOB71_RS22085 Genome accession   NZ_CP044069
Coordinates   3033975..3034565 (-) Length   196 a.a.
NCBI ID   WP_017419657.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FDAARGOS_663     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3028975..3039565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB71_RS22060 (FOB71_22055) aroB 3029210..3030301 (-) 1092 WP_011079358.1 3-dehydroquinate synthase -
  FOB71_RS22065 (FOB71_22060) aroK 3030334..3030852 (-) 519 WP_011079357.1 shikimate kinase AroK -
  FOB71_RS22070 (FOB71_22065) pilQ 3031092..3032876 (-) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  FOB71_RS22075 (FOB71_22070) pilP 3032890..3033402 (-) 513 WP_017419659.1 pilus assembly protein PilP Machinery gene
  FOB71_RS22080 (FOB71_22075) pilO 3033392..3033982 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FOB71_RS22085 (FOB71_22080) pilN 3033975..3034565 (-) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  FOB71_RS22090 (FOB71_22085) pilM 3034555..3035562 (-) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FOB71_RS22095 (FOB71_22090) - 3035702..3038215 (+) 2514 WP_017419655.1 penicillin-binding protein 1A -
  FOB71_RS22100 (FOB71_22095) oxyR 3038284..3039183 (-) 900 WP_026050199.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22644.86 Da        Isoelectric Point: 7.6441

>NTDB_id=387401 FOB71_RS22085 WP_017419657.1 3033975..3034565(-) (pilN) [Vibrio vulnificus strain FDAARGOS_663]
MQHKINLLPWREHKREEHKRRFVSLVALGALIAVGVQWGIGQYFSYQQRLQQERLDYLTHYIAQLDQRIQAMNIAKEEHS
QILARLKVVESLQAERNKTTAFMNLMTELIPEGVYVDKIKMNTNDVELAGISDSTARLATMLDNLERSSFLKEVEMHSIV
HDKKRFGKSFQTFTVSFVFDEHADSANTLAKEGNHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=387401 FOB71_RS22085 WP_017419657.1 3033975..3034565(-) (pilN) [Vibrio vulnificus strain FDAARGOS_663]
ATGCAGCATAAGATCAACCTGTTACCTTGGCGTGAACACAAGCGTGAGGAGCATAAACGTCGTTTTGTCAGTCTGGTCGC
ATTAGGTGCCTTGATCGCGGTCGGTGTTCAGTGGGGCATTGGCCAGTACTTTTCATACCAACAACGTTTGCAGCAAGAGC
GTCTCGATTATCTTACCCACTATATCGCACAGTTGGATCAGCGTATTCAAGCGATGAACATTGCCAAGGAAGAGCATAGC
CAGATTCTCGCTCGACTCAAGGTTGTCGAGTCGCTTCAAGCGGAGCGCAACAAAACCACCGCATTTATGAACTTGATGAC
GGAGCTGATCCCAGAGGGGGTGTATGTCGACAAGATCAAAATGAACACCAACGACGTTGAGTTGGCAGGTATAAGCGATA
GTACCGCTCGACTCGCCACCATGTTGGATAATTTGGAACGTTCCTCCTTCCTGAAAGAAGTGGAAATGCATTCGATTGTT
CATGACAAGAAGCGTTTTGGTAAGAGCTTCCAGACGTTTACCGTCTCTTTTGTGTTTGATGAACATGCCGATTCTGCCAA
TACGTTGGCCAAGGAGGGGAATCATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

68.508

92.347

0.633
  pilN Vibrio cholerae strain A1552

63.277

90.306

0.571

Multiple sequence alignment