Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FOB71_RS22090 Genome accession   NZ_CP044069
Coordinates   3034555..3035562 (-) Length   335 a.a.
NCBI ID   WP_017419656.1    Uniprot ID   A0A087I1V6
Organism   Vibrio vulnificus strain FDAARGOS_663     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3029555..3040562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB71_RS22065 (FOB71_22060) aroK 3030334..3030852 (-) 519 WP_011079357.1 shikimate kinase AroK -
  FOB71_RS22070 (FOB71_22065) pilQ 3031092..3032876 (-) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  FOB71_RS22075 (FOB71_22070) pilP 3032890..3033402 (-) 513 WP_017419659.1 pilus assembly protein PilP Machinery gene
  FOB71_RS22080 (FOB71_22075) pilO 3033392..3033982 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FOB71_RS22085 (FOB71_22080) pilN 3033975..3034565 (-) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  FOB71_RS22090 (FOB71_22085) pilM 3034555..3035562 (-) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FOB71_RS22095 (FOB71_22090) - 3035702..3038215 (+) 2514 WP_017419655.1 penicillin-binding protein 1A -
  FOB71_RS22100 (FOB71_22095) oxyR 3038284..3039183 (-) 900 WP_026050199.1 DNA-binding transcriptional regulator OxyR -
  FOB71_RS22105 (FOB71_22100) - 3039457..3039729 (+) 273 WP_017419653.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37654.05 Da        Isoelectric Point: 6.3873

>NTDB_id=387402 FOB71_RS22090 WP_017419656.1 3034555..3035562(-) (pilM) [Vibrio vulnificus strain FDAARGOS_663]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=387402 FOB71_RS22090 WP_017419656.1 3034555..3035562(-) (pilM) [Vibrio vulnificus strain FDAARGOS_663]
ATGGGTAAACCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAGTTGCCTCTTTTCCGTTATCAGGTGTCACTGGCGGTACCCGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAACGAGAGTTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGAGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTACATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGACAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCACCTTTGGTTAAAGAGTTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAGCTGACAGAGCGAGTTCGTCGGCAATTGCAGTTGTTCCAATCCATGGATAGCCAAAGCAAAGTGAATGGCCTTTGGAT
CTGCGGTGAAGGCGCGATTACGCCGTTGTTGAGTGAAACTTTGCAGAGAAAGCTGAGTTTGGAGTGTGAAGTCCTTAATC
CTCTGGCTCTATTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTCGCCGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087I1V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment