Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HHJ35_RS03560 Genome accession   NZ_CP051714
Coordinates   731858..732517 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain SCU-122     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 726858..737517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ35_RS03535 (HHJ35_03535) ygiN 727705..728019 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  HHJ35_RS03540 (HHJ35_03540) mdaB 728050..728631 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HHJ35_RS24365 - 728881..729414 (+) 534 WP_002430503.1 Hcp family type VI secretion system effector -
  HHJ35_RS24370 - 729417..730127 (+) 711 WP_000834021.1 hypothetical protein -
  HHJ35_RS03550 (HHJ35_03550) ygiZ 730134..730466 (+) 333 WP_000914696.1 DUF2645 family protein -
  HHJ35_RS03555 (HHJ35_03555) qseC 730512..731861 (-) 1350 WP_000673382.1 quorum sensing histidine kinase QseC -
  HHJ35_RS03560 (HHJ35_03560) ciaR 731858..732517 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  HHJ35_RS03565 (HHJ35_03565) ygiW 732669..733061 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HHJ35_RS03570 (HHJ35_03570) ygiV 733114..733596 (+) 483 WP_000183493.1 GyrI-like domain-containing protein -
  HHJ35_RS03575 (HHJ35_03575) ygiS 733705..735312 (+) 1608 WP_001350728.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=387137 HHJ35_RS03560 WP_001221493.1 731858..732517(-) (ciaR) [Escherichia coli strain SCU-122]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=387137 HHJ35_RS03560 WP_001221493.1 731858..732517(-) (ciaR) [Escherichia coli strain SCU-122]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365