Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   HHM37_RS12515 Genome accession   NZ_CP051609
Coordinates   2615108..2617189 (+) Length   693 a.a.
NCBI ID   WP_000678405.1    Uniprot ID   -
Organism   Escherichia coli O25b:H4-ST131 strain 2019_APHA     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2610108..2622189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHM37_RS12495 (HHM37_12490) gmk 2611326..2611949 (+) 624 WP_001295237.1 guanylate kinase -
  HHM37_RS12500 (HHM37_12495) rpoZ 2612004..2612279 (+) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  HHM37_RS12505 (HHM37_12500) spoT 2612298..2614406 (+) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  HHM37_RS12510 (HHM37_12505) trmH 2614413..2615102 (+) 690 WP_001070179.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  HHM37_RS12515 (HHM37_12510) recG 2615108..2617189 (+) 2082 WP_000678405.1 ATP-dependent DNA helicase RecG Machinery gene
  HHM37_RS12520 (HHM37_12515) gltS 2617223..2618428 (-) 1206 WP_000468826.1 sodium/glutamate symporter -
  HHM37_RS12525 (HHM37_12520) xanP 2618708..2620099 (+) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  HHM37_RS12530 (HHM37_12525) yicH 2620220..2621929 (+) 1710 WP_001296535.1 AsmA family protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76524.50 Da        Isoelectric Point: 7.3384

>NTDB_id=385637 HHM37_RS12515 WP_000678405.1 2615108..2617189(+) (recG) [Escherichia coli O25b:H4-ST131 strain 2019_APHA]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=385637 HHM37_RS12515 WP_000678405.1 2615108..2617189(+) (recG) [Escherichia coli O25b:H4-ST131 strain 2019_APHA]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCATACCGTACAGGATTTGCTCTTACACCTTCCTCTGCGCTACGAAGATCGCACACATCTCTACCCCATCG
GTGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACTATGCGCTTTTTCAACTTCAATGCGGCAATGAAAAATAGCCT
GGCGACAGGCCGCCGCGTGCTGGCCTATGGCGAAGCAAAGCGCGGCAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTACAGGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCTACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACATGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCACCGCCGACGCTACAGCTTAGCGATCTCG
AAACCGGGCAGCATCCGGCACAACGCCGGCTGATTCTGGAAGAGTTGCTGGCGCACAACCTCAGCATGTTGGCGTTACGT
GCCGGTGCACAACGTTTTCATGCTCAGCCGCTGAGCGCCAATGACGCGCTGAAAAATAAACTCCTCGCCGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGG
TGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCGCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTAGGCTGGCTCGCCGGAAAACAGAAAGGTAAAGCACGACTGGCTCAGCAAGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTTGGTACGCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCCATCCCCCGCACGCTGGCGATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCACCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGTATG
ACTGAAGGGCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTACTGGAAGCGCAGGCGGCGGAAGCGAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAATGTTGGCCTGGTACACGGACGGATGAAACCCGCCGAGAAACAGG
CGGTGATGACATCGTTTAAACAAGGCGAGCTACACCTGCTGGTTGCCACTACTGTCATTGAAGTCGGTGTTGATGTACCC
AATGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATCCGCCTGCAGGTACTAC
GCGACAGCAACGATGGTTTTGTGATTGCACAGAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGTACACGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.21

94.949

0.372