Detailed information NTG0038496

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BAMF_RS32905
• Genome accession: NC_014551
• Coordinates: 2503187..2503801 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens DSM 7 = ATCC 23350
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2498187..2508801

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAMF_RS32880 (BAMF_2452)	rpsT	2498487..2498753 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BAMF_RS32885 (BAMF_2453)	holA	2498770..2499811 (-)	1042	Protein_2516	DNA polymerase III subunit delta	-
BAMF_RS41320	-	2499851..2500005 (-)	155	Protein_2517	hypothetical protein	-
BAMF_RS40855 (BAMF_2454)	-	2500046..2500180 (+)	135	WP_003152870.1	YqzM family protein	-
BAMF_RS32895 (BAMF_2455)	comEC	2500199..2502550 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BAMF_RS32900 (BAMF_2456)	-	2502551..2503120 (-)	570	WP_003152868.1	ComE operon protein 2	-
BAMF_RS32905 (BAMF_2457)	comEA	2503187..2503801 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
BAMF_RS32910 (BAMF_2458)	comER	2503860..2504681 (+)	822	WP_013352943.1	late competence protein ComER	-
BAMF_RS32915 (BAMF_2459)	-	2504750..2505487 (-)	738	WP_013352944.1	class I SAM-dependent DNA methyltransferase	-
BAMF_RS32920 (BAMF_2460)	rsfS	2505484..2505840 (-)	357	WP_003152864.1	ribosome silencing factor	-
BAMF_RS32925 (BAMF_2461)	yqeK	2505858..2506418 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BAMF_RS32930 (BAMF_2462)	-	2506408..2506977 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
BAMF_RS32935 (BAMF_2463)	yhbY	2506988..2507278 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BAMF_RS32940 (BAMF_2464)	aroE	2507272..2508114 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=38496 BAMF_RS32905 WP_013352942.1 2503187..2503801(-) (comEA) [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=38496 BAMF_RS32905 WP_013352942.1 2503187..2503801(-) (comEA) [Bacillus amyloliquefaciens DSM 7 = ATCC 23350]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363