Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FZE25_RS11980 Genome accession   NZ_CP043416
Coordinates   2447669..2448739 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain ONU 553     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442669..2453739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZE25_RS11930 (FZE25_11725) sipW 2442729..2443313 (-) 585 WP_007408328.1 signal peptidase I SipW -
  FZE25_RS11935 (FZE25_11730) tapA 2443285..2443956 (-) 672 WP_042635356.1 amyloid fiber anchoring/assembly protein TapA -
  FZE25_RS11940 (FZE25_11735) - 2444215..2444544 (+) 330 WP_020954231.1 DUF3889 domain-containing protein -
  FZE25_RS11945 (FZE25_11740) - 2444584..2444763 (-) 180 WP_003153093.1 YqzE family protein -
  FZE25_RS11950 (FZE25_11745) comGG 2444820..2445197 (-) 378 WP_032866434.1 competence type IV pilus minor pilin ComGG Machinery gene
  FZE25_RS11955 (FZE25_11750) comGF 2445198..2445698 (-) 501 WP_262982688.1 competence type IV pilus minor pilin ComGF -
  FZE25_RS11960 (FZE25_11755) comGE 2445607..2445921 (-) 315 WP_007408323.1 competence type IV pilus minor pilin ComGE -
  FZE25_RS11965 (FZE25_11760) comGD 2445905..2446342 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  FZE25_RS11970 (FZE25_11765) comGC 2446332..2446640 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  FZE25_RS11975 (FZE25_11770) comGB 2446645..2447682 (-) 1038 WP_032866436.1 competence type IV pilus assembly protein ComGB Machinery gene
  FZE25_RS11980 (FZE25_11775) comGA 2447669..2448739 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  FZE25_RS11985 (FZE25_11780) - 2448931..2449881 (-) 951 WP_032870601.1 magnesium transporter CorA family protein -
  FZE25_RS11990 (FZE25_11785) - 2450027..2451328 (+) 1302 WP_012117986.1 hemolysin family protein -
  FZE25_RS11995 (FZE25_11790) - 2451366..2452196 (-) 831 WP_007408317.1 STAS domain-containing protein -
  FZE25_RS12005 (FZE25_11800) - 2452403..2452783 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FZE25_RS12010 (FZE25_11805) - 2453020..2453265 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=383691 FZE25_RS11980 WP_012117985.1 2447669..2448739(-) (comGA) [Bacillus velezensis strain ONU 553]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=383691 FZE25_RS11980 WP_012117985.1 2447669..2448739(-) (comGA) [Bacillus velezensis strain ONU 553]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCCTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment