Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   P411_RS18865 Genome accession   NZ_CP050998
Coordinates   3735508..3736167 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O39:NM str. F8704-2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3730508..3741167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P411_RS18840 parE 3730599..3732491 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  P411_RS18845 ygiN 3732539..3732853 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  P411_RS18850 mdaB 3732884..3733465 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  P411_RS18855 ygiZ 3733784..3734116 (+) 333 WP_000912119.1 DUF2645 family protein -
  P411_RS18860 qseC 3734162..3735511 (-) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  P411_RS18865 ciaR 3735508..3736167 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  P411_RS18870 ygiW 3736319..3736711 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  P411_RS18875 ygiV 3736764..3737246 (+) 483 WP_032361374.1 GyrI-like domain-containing protein -
  P411_RS18880 parC 3737792..3740050 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  P411_RS18885 plsC 3740283..3741020 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=382437 P411_RS18865 WP_001221502.1 3735508..3736167(-) (ciaR) [Escherichia coli O39:NM str. F8704-2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=382437 P411_RS18865 WP_001221502.1 3735508..3736167(-) (ciaR) [Escherichia coli O39:NM str. F8704-2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment