Detailed information NTG0038205

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BSUW23_RS12870
• Genome accession: NC_014479
• Coordinates: 2496490..2497107 (-)
• Length: 205 a.a.
• NCBI ID: WP_003226145.1
• Uniprot ID: -
• Organism: Bacillus spizizenii str. W23
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2491490..2502107

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSUW23_RS12845 (BSUW23_12665)	rpsT	2491791..2492057 (+)	267	WP_003226155.1	30S ribosomal protein S20	-
BSUW23_RS12850 (BSUW23_12670)	holA	2492072..2493115 (-)	1044	WP_003226154.1	DNA polymerase III subunit delta	-
BSUW23_RS21300	-	2493155..2493304 (-)	150	WP_136975859.1	hypothetical protein	-
BSUW23_RS12855 (BSUW23_12680)	-	2493345..2493479 (+)	135	WP_003226152.1	YqzM family protein	-
BSUW23_RS12860 (BSUW23_12685)	comEC	2493520..2495850 (-)	2331	WP_003226149.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BSUW23_RS12865 (BSUW23_12690)	-	2495854..2496423 (-)	570	WP_003226147.1	ComE operon protein 2	-
BSUW23_RS12870 (BSUW23_12695)	comEA	2496490..2497107 (-)	618	WP_003226145.1	helix-hairpin-helix domain-containing protein	Machinery gene
BSUW23_RS12875 (BSUW23_12700)	comER	2497191..2498012 (+)	822	WP_003226143.1	late competence protein ComER	-
BSUW23_RS12880 (BSUW23_12705)	-	2498078..2498821 (-)	744	WP_003226141.1	class I SAM-dependent methyltransferase	-
BSUW23_RS12885 (BSUW23_12710)	rsfS	2498818..2499174 (-)	357	WP_003226139.1	ribosome silencing factor	-
BSUW23_RS12890 (BSUW23_12715)	yqeK	2499192..2499752 (-)	561	WP_003226137.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BSUW23_RS12895 (BSUW23_12720)	-	2499742..2500311 (-)	570	WP_003226135.1	nicotinate-nucleotide adenylyltransferase	-
BSUW23_RS12900 (BSUW23_12725)	yhbY	2500323..2500613 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
BSUW23_RS12905 (BSUW23_12730)	aroE	2500607..2501449 (-)	843	WP_003226130.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21919.78 Da
• Isoelectric Point: 5.1470

>NTDB_id=38205 BSUW23_RS12870 WP_003226145.1 2496490..2497107(-) (comEA) [Bacillus spizizenii str. W23]
MNWLNHHKKAIILGASAAVFIAIIFFLASGKNKESMKQTVSTETENTEVKQEAVKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAGLLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=38205 BSUW23_RS12870 WP_003226145.1 2496490..2497107(-) (comEA) [Bacillus spizizenii str. W23]
ATGAATTGGTTGAATCATCATAAGAAAGCAATTATTTTAGGGGCCTCTGCGGCTGTTTTCATAGCGATCATTTTCTTCCT
GGCCAGCGGGAAAAACAAAGAGTCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAG
TCAAAGATGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCACCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCCCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGG
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGAAAGGGGGCGCTGGTAAATATCAATACAGCAACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAATTGAAGATATTACGAAGGT
TTCAGGGATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	89.268	100	0.893
comEA	Latilactobacillus sakei subsp. sakei 23K	31.646	100	0.366
comEA	Staphylococcus aureus MW2	37.688	97.073	0.366