Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BMF77_RS19395 Genome accession   NZ_CP043056
Coordinates   4040752..4042752 (+) Length   666 a.a.
NCBI ID   WP_148765083.1    Uniprot ID   A0A5C0DV56
Organism   Dolichospermum sp. UHCC 0315A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4035752..4047752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMF77_RS19375 (BMF77_03775) dnaK 4037030..4038889 (-) 1860 WP_148765079.1 molecular chaperone DnaK -
  BMF77_RS29065 - 4039165..4039332 (+) 168 WP_371735126.1 hypothetical protein -
  BMF77_RS19390 (BMF77_03776) grpE 4039504..4040178 (-) 675 WP_148765081.1 nucleotide exchange factor GrpE -
  BMF77_RS19395 (BMF77_03777) pilB/pilB1 4040752..4042752 (+) 2001 WP_148765083.1 GspE/PulE family protein Machinery gene
  BMF77_RS19400 (BMF77_03778) pilT 4042817..4043917 (+) 1101 WP_148765085.1 type IV pilus twitching motility protein PilT Machinery gene
  BMF77_RS26875 - 4043909..4044058 (-) 150 WP_168204467.1 hypothetical protein -
  BMF77_RS19405 (BMF77_03779) - 4044090..4045313 (+) 1224 WP_148765087.1 type II secretion system F family protein -
  BMF77_RS19410 (BMF77_03780) - 4045507..4047399 (+) 1893 WP_148765089.1 GTPase family protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74552.51 Da        Isoelectric Point: 6.2786

>NTDB_id=380413 BMF77_RS19395 WP_148765083.1 4040752..4042752(+) (pilB/pilB1) [Dolichospermum sp. UHCC 0315A]
MTYSSPQRRSTALTTRTEFSPFGNKLVTSGYVNNEQMKQAVIASRKSGKPLTEVLESITERKLSPELVTEYKKQHLFELK
ILYGLESLDPEIYEIDTTNVGNLIQTLIPVDICRRHHLIPLSRNDNQSPPYVLVAMVDPDNLDASDDLNRILRSQGLSFQ
RRVITQKDYQDIINKFLNEQAARRKILDQQKNYDVKGDLDNLGTLDIDESSDNDVDLDNAMKGADHAPIIKLVNRILLKA
LTEGVSDIHIEPQEEYLRIRFRKDGVLHEAFDNMPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRIFAGRKVDFRVNT
LPSRYGEKVVVRILDNSATQLGLDKLITDPETLNIVKDMFSKPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVED
PIEYSLPGITQVQVIREKGLDFSTVLRAFLRQDPDVLLVGETRDRETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGEM
GIEPFMISSSLIGVLAQRLVRRVCAECRIAYTPTIKELARYGLSVSQEAQVTFYKANTIPPEEIASHKNLCQKCGGVGYK
GRCGVYEVMRITEELQTLINKQAPTEKIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTLTDKGLEAELKAKRKSGL
TCLTCSGELQPEWLDCPYCMTPCFTD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=380413 BMF77_RS19395 WP_148765083.1 4040752..4042752(+) (pilB/pilB1) [Dolichospermum sp. UHCC 0315A]
ATGACTTACTCATCACCACAACGGCGTAGCACTGCGCTGACTACAAGAACTGAGTTTTCGCCTTTCGGTAATAAATTAGT
AACGTCTGGCTATGTCAATAATGAACAAATGAAGCAGGCTGTAATTGCCAGCCGCAAGTCCGGTAAACCACTGACAGAAG
TATTAGAATCAATTACTGAGCGAAAATTATCACCTGAGTTGGTCACCGAATACAAAAAACAACATTTATTTGAACTCAAA
ATTTTATACGGCCTTGAGTCTCTTGATCCCGAAATCTACGAAATTGATACAACAAACGTGGGGAATTTAATTCAAACCCT
CATTCCTGTTGATATCTGTCGTCGGCATCACTTAATCCCACTGTCAAGAAATGATAACCAAAGCCCACCTTATGTGTTGG
TGGCTATGGTTGATCCAGATAATTTAGATGCTTCTGATGATCTTAATCGGATTTTGCGTTCCCAAGGCTTGTCTTTTCAA
CGCAGGGTAATTACCCAGAAAGATTATCAGGACATCATTAACAAATTTTTAAATGAACAAGCGGCACGCCGAAAGATATT
AGACCAGCAAAAAAATTATGATGTCAAGGGTGATTTAGACAATCTAGGGACTCTGGACATAGATGAAAGTAGTGATAATG
ATGTTGATTTAGATAATGCTATGAAGGGTGCTGATCATGCACCAATCATTAAACTTGTGAATCGGATTTTGTTGAAGGCT
TTAACTGAGGGTGTTTCGGATATTCACATTGAACCGCAGGAAGAATATTTACGGATTCGTTTCCGTAAGGATGGGGTATT
ACATGAGGCATTTGATAATATGCCGAAAAAAATTATCCCAGCGGTAACAGCCCGATTTAAAATTATTTCTAACCTGGACA
TTGCTGAAAGACGTTTACCTCAAGATGGCCGGATTCGCCGGATATTTGCCGGAAGAAAGGTGGATTTCCGGGTAAATACT
CTACCTAGTCGCTATGGAGAAAAGGTTGTTGTACGGATTTTAGATAACTCTGCAACTCAATTAGGTTTGGATAAGTTAAT
TACTGACCCAGAAACCTTAAATATTGTTAAGGATATGTTTAGTAAGCCATTCGGGTTGATTTTAGTGACGGGACCAACTG
GTTCGGGTAAAACTACTTCGTTGTATTCGGCACTATCGGAAAAGAATGATCCGGGGATTAATATCAGTACAGTAGAAGAT
CCAATTGAGTATAGTTTACCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAGGGGTTGGATTTCTCGACGGTCTTGCG
GGCTTTCTTGCGTCAAGATCCAGATGTGTTGCTGGTGGGGGAAACACGGGATCGGGAAACTGCGAAAACGGCGATTGAGG
CAGCATTAACCGGTCACTTGGTATTAACTACTTTACACACTAATGATGCACCAGGAGCGATCGCTCGCTTGGGAGAAATG
GGTATTGAACCTTTCATGATTTCCAGTTCTTTAATTGGTGTTTTAGCACAACGTCTAGTTCGTCGTGTCTGTGCTGAATG
TCGTATAGCATACACTCCGACCATTAAAGAACTAGCTCGCTATGGTCTATCCGTTTCCCAAGAAGCTCAGGTAACTTTCT
ACAAAGCCAATACTATCCCACCAGAAGAAATAGCCAGCCATAAAAATCTTTGTCAAAAATGTGGTGGTGTTGGCTACAAG
GGGCGTTGTGGGGTTTATGAAGTAATGCGGATTACTGAAGAATTACAAACTCTGATCAACAAGCAAGCACCTACAGAAAA
GATTAAAGAAGTAGCTGTTGAAGAGGGGATGAAAACCTTACTAGCTTACAGTTTGGACTTAGTTCGTCAAGGAGCAACTA
CCTTAGAAGAAGTAGAGCGAGTAACACTTACAGATAAAGGTTTAGAGGCTGAATTAAAAGCTAAACGCAAAAGTGGTCTG
ACTTGTCTGACTTGTAGTGGGGAATTACAACCAGAATGGTTAGATTGTCCTTACTGTATGACACCTTGCTTTACAGATTA
G

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-181)

Type II/IV secretion system protein (T2SSE)

(235-502)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0DV56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

63.501

100

0.643

Multiple sequence alignment