Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FXN59_RS03570 Genome accession   NZ_CP043004
Coordinates   657960..658412 (+) Length   150 a.a.
NCBI ID   WP_148327474.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain HK_973     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 652960..663412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN59_RS03555 (FXN59_03555) pnp 653918..656047 (-) 2130 WP_148323370.1 polyribonucleotide nucleotidyltransferase -
  FXN59_RS03560 (FXN59_03560) nanQ 656340..656810 (+) 471 WP_148323372.1 N-acetylneuraminate anomerase -
  FXN59_RS03565 (FXN59_03565) ampD 657284..657838 (-) 555 WP_148323374.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FXN59_RS03570 (FXN59_03570) pilA 657960..658412 (+) 453 WP_148327474.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FXN59_RS03575 (FXN59_03575) - 658439..659726 (+) 1288 Protein_689 GspE/PulE family protein -
  FXN59_RS03580 (FXN59_03580) - 659728..660228 (+) 501 Protein_690 type II secretion system F family protein -
  FXN59_RS12405 (FXN59_03585) - 660225..660431 (+) 207 Protein_691 prepilin peptidase -
  FXN59_RS03590 (FXN59_03590) - 660439..661257 (+) 819 WP_262404593.1 DUF4123 domain-containing protein -
  FXN59_RS03595 (FXN59_03595) - 661280..662728 (+) 1449 Protein_693 hypothetical protein -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15532.99 Da        Isoelectric Point: 9.6572

>NTDB_id=380137 FXN59_RS03570 WP_148327474.1 657960..658412(+) (pilA) [Aggregatibacter actinomycetemcomitans strain HK_973]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSKLLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGAITVTGKGSLDKISYTLTASGTAATGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=380137 FXN59_RS03570 WP_148327474.1 657960..658412(+) (pilA) [Aggregatibacter actinomycetemcomitans strain HK_973]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATAGC
TATCGTTGCTATTCTCGCCACCGTAGCCGTGCCATCTTATCAAAATTACACCAAAAAAGCGGCGGTTTCCAAATTATTAC
AAGCCTCCGCGCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AATGGCATTCAAGCAGATGTTGCGGATGCTTCGAAACAAAAATATGTGAAATCCACCGCGGTCAAGGGTGGTGCGATCAC
TGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGGCACAGCTGCCACCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.043

92

0.58
  pilA Haemophilus influenzae Rd KW20

58.333

96

0.56
  pilA Glaesserella parasuis strain SC1401

52.448

95.333

0.5

Multiple sequence alignment