Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FXN58_RS00995 Genome accession   NZ_CP043003
Coordinates   201275..201727 (+) Length   150 a.a.
NCBI ID   WP_148323376.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain HK_907     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196275..206727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN58_RS00980 (FXN58_00980) pnp 197233..199362 (-) 2130 WP_148323370.1 polyribonucleotide nucleotidyltransferase -
  FXN58_RS00985 (FXN58_00985) nanQ 199655..200125 (+) 471 WP_148323372.1 N-acetylneuraminate anomerase -
  FXN58_RS00990 (FXN58_00990) ampD 200599..201153 (-) 555 WP_148323374.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FXN58_RS00995 (FXN58_00995) pilA 201275..201727 (+) 453 WP_148323376.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FXN58_RS01000 (FXN58_01000) - 201754..202572 (+) 819 Protein_195 GspE/PulE family protein -
  FXN58_RS01020 (FXN58_01020) - 203188..203448 (-) 261 WP_148323380.1 DNA-binding protein -
  FXN58_RS01025 (FXN58_01025) - 203638..203850 (+) 213 WP_032996596.1 helix-turn-helix domain-containing protein -
  FXN58_RS01030 (FXN58_01030) - 203866..204312 (+) 447 WP_262404476.1 DNA-binding protein -
  FXN58_RS01035 (FXN58_01035) - 204296..204715 (+) 420 Protein_199 putative quinol monooxygenase -
  FXN58_RS01040 (FXN58_01040) secE 205152..205562 (+) 411 WP_148323382.1 preprotein translocase subunit SecE -
  FXN58_RS01045 (FXN58_01045) nusG 205564..206115 (+) 552 WP_005547787.1 transcription termination/antitermination protein NusG -
  FXN58_RS01050 (FXN58_01050) rplK 206272..206700 (+) 429 WP_148323384.1 50S ribosomal protein L11 -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15591.97 Da        Isoelectric Point: 8.8052

>NTDB_id=380110 FXN58_RS00995 WP_148323376.1 201275..201727(+) (pilA) [Aggregatibacter actinomycetemcomitans strain HK_907]
MKIQSAFISLKFVKKGFTLIELMIVIAIVAILATVAVPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGKAENCSGGQ
NGIQADVADASKQKYVKSTAVKGGAITVTGKGSLDKISYTLTASDTAATGVSWNAACGNNADIFPAGFCS

Nucleotide


Download         Length: 453 bp        

>NTDB_id=380110 FXN58_RS00995 WP_148323376.1 201275..201727(+) (pilA) [Aggregatibacter actinomycetemcomitans strain HK_907]
ATGAAAATTCAATCCGCGTTTATTTCGCTAAAATTCGTTAAAAAAGGGTTTACATTAATCGAATTAATGATCGTCATAGC
TATCGTTGCTATTCTCGCCACCGTAGCCGTGCCATCTTATCAAAATTACACCAAAAAAGCGGCGGTTTCCGAATTATTAC
AAGCCTCCGCGCCATTACGTTCCGAAGTGGAACTCTGTATTTATAACACCGGTAAGGCTGAAAATTGTAGCGGCGGACAA
AATGGCATTCAAGCAGATGTTGCGGATGCTTCGAAACAAAAATATGTGAAATCCACCGCGGTCAAGGGTGGTGCGATCAC
TGTTACCGGCAAAGGCAGTTTGGATAAAATCAGCTACACCCTTACCGCTTCGGACACAGCTGCCACCGGGGTCAGCTGGA
ACGCCGCCTGCGGCAATAATGCCGACATTTTCCCGGCAGGTTTCTGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.043

92

0.58
  pilA Haemophilus influenzae Rd KW20

58.333

96

0.56
  pilA Glaesserella parasuis strain SC1401

53.147

95.333

0.507

Multiple sequence alignment