Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HB730_RS29910 Genome accession   NZ_CP050332
Coordinates   6343264..6343887 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain DVT413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6338264..6348887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB730_RS29890 (HB730_29980) aroB 6338859..6339965 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  HB730_RS29895 (HB730_29985) aroK 6340015..6340533 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HB730_RS29900 (HB730_29990) pilQ 6340545..6342689 (-) 2145 WP_058176333.1 type 4a pilus secretin PilQ Machinery gene
  HB730_RS29905 (HB730_29995) comL/pilP 6342743..6343267 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HB730_RS29910 (HB730_30000) pilO 6343264..6343887 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB730_RS29915 (HB730_30005) pilN 6343884..6344480 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HB730_RS29920 (HB730_30010) pilM 6344480..6345544 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HB730_RS29925 (HB730_30015) - 6345729..6348200 (+) 2472 WP_019484455.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=379113 HB730_RS29910 WP_003103268.1 6343264..6343887(-) (pilO) [Pseudomonas aeruginosa strain DVT413]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=379113 HB730_RS29910 WP_003103268.1 6343264..6343887(-) (pilO) [Pseudomonas aeruginosa strain DVT413]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401