Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HB731_RS27535 Genome accession   NZ_CP050331
Coordinates   5930635..5931258 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain DVT414     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5925635..5936258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB731_RS27515 (HB731_27555) aroB 5926230..5927336 (-) 1107 WP_003116138.1 3-dehydroquinate synthase -
  HB731_RS27520 (HB731_27560) aroK 5927386..5927904 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HB731_RS27525 (HB731_27565) pilQ 5927916..5930060 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  HB731_RS27530 (HB731_27570) comL/pilP 5930114..5930638 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  HB731_RS27535 (HB731_27575) pilO 5930635..5931258 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB731_RS27540 (HB731_27580) pilN 5931255..5931851 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  HB731_RS27545 (HB731_27585) pilM 5931851..5932915 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HB731_RS27550 (HB731_27590) - 5933100..5935571 (+) 2472 WP_016562439.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=379014 HB731_RS27535 WP_003103268.1 5930635..5931258(-) (pilO) [Pseudomonas aeruginosa strain DVT414]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=379014 HB731_RS27535 WP_003103268.1 5930635..5931258(-) (pilO) [Pseudomonas aeruginosa strain DVT414]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTGAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401