Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FR729_RS12185 Genome accession   NZ_CP042449
Coordinates   2592527..2593543 (-) Length   338 a.a.
NCBI ID   WP_005382774.1    Uniprot ID   A0AAP5Z0M3
Organism   Vibrio alginolyticus strain FA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2587527..2598543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR729_RS12160 (FR729_12530) aroK 2588400..2588918 (-) 519 WP_005381319.1 shikimate kinase AroK -
  FR729_RS12165 (FR729_12535) pilQ 2589106..2590851 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  FR729_RS12170 (FR729_12540) pilP 2590877..2591392 (-) 516 WP_167415487.1 pilus assembly protein PilP Machinery gene
  FR729_RS12175 (FR729_12545) pilO 2591382..2591975 (-) 594 WP_005382767.1 type 4a pilus biogenesis protein PilO Machinery gene
  FR729_RS12180 (FR729_12550) pilN 2591968..2592543 (-) 576 WP_167415488.1 PilN domain-containing protein Machinery gene
  FR729_RS12185 (FR729_12555) pilM 2592527..2593543 (-) 1017 WP_005382774.1 type IV pilus biogenesis protein PilM Machinery gene
  FR729_RS12190 (FR729_12560) - 2593688..2596249 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  FR729_RS12195 (FR729_12565) oxyR 2596302..2597213 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  FR729_RS12200 (FR729_12570) - 2597383..2598111 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37609.97 Da        Isoelectric Point: 7.2224

>NTDB_id=377954 FR729_RS12185 WP_005382774.1 2592527..2593543(-) (pilM) [Vibrio alginolyticus strain FA2]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=377954 FR729_RS12185 WP_005382774.1 2592527..2593543(-) (pilM) [Vibrio alginolyticus strain FA2]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGTGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTTGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTGGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment