Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   FR729_RS12180 Genome accession   NZ_CP042449
Coordinates   2591968..2592543 (-) Length   191 a.a.
NCBI ID   WP_167415488.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2586968..2597543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR729_RS12155 (FR729_12525) aroB 2587267..2588367 (-) 1101 WP_005382764.1 3-dehydroquinate synthase -
  FR729_RS12160 (FR729_12530) aroK 2588400..2588918 (-) 519 WP_005381319.1 shikimate kinase AroK -
  FR729_RS12165 (FR729_12535) pilQ 2589106..2590851 (-) 1746 WP_005382765.1 type IV pilus secretin PilQ Machinery gene
  FR729_RS12170 (FR729_12540) pilP 2590877..2591392 (-) 516 WP_167415487.1 pilus assembly protein PilP Machinery gene
  FR729_RS12175 (FR729_12545) pilO 2591382..2591975 (-) 594 WP_005382767.1 type 4a pilus biogenesis protein PilO Machinery gene
  FR729_RS12180 (FR729_12550) pilN 2591968..2592543 (-) 576 WP_167415488.1 PilN domain-containing protein Machinery gene
  FR729_RS12185 (FR729_12555) pilM 2592527..2593543 (-) 1017 WP_005382774.1 type IV pilus biogenesis protein PilM Machinery gene
  FR729_RS12190 (FR729_12560) - 2593688..2596249 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  FR729_RS12195 (FR729_12565) oxyR 2596302..2597213 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22348.83 Da        Isoelectric Point: 7.4657

>NTDB_id=377953 FR729_RS12180 WP_167415488.1 2591968..2592543(-) (pilN) [Vibrio alginolyticus strain FA2]
MLYRINLLPWRENQREEHRRRFISLVALGMLVAVGIQWGVGKFFEYQQDQQQDRLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=377953 FR729_RS12180 WP_167415488.1 2591968..2592543(-) (pilN) [Vibrio alginolyticus strain FA2]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAATCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTGTTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGATC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGTCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCAGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

62.712

92.67

0.581

Multiple sequence alignment