Detailed information NTG0377065

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HBA47_RS08105
• Genome accession: NZ_CP050136
• Coordinates: 1534078..1535358 (-)
• Length: 426 a.a.
• NCBI ID: WP_003787551.1
• Uniprot ID: F5S877
• Organism: Kingella kingae strain ATCC 23332
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1529078..1540358

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBA47_RS08080 (HBA47_08115)	folD	1530096..1530947 (+)	852	WP_003791096.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-
HBA47_RS08085 (HBA47_08120)	-	1531090..1532478 (+)	1389	WP_019389593.1	GntP family permease	-
HBA47_RS08090 (HBA47_08125)	-	1532603..1533112 (+)	510	WP_154700278.1	gluconokinase	-
HBA47_RS08095 (HBA47_08130)	-	1533183..1533560 (-)	378	WP_019389591.1	diacylglycerol kinase	-
HBA47_RS08100 (HBA47_08135)	-	1533557..1534000 (-)	444	WP_019389590.1	peroxiredoxin	-
HBA47_RS08105 (HBA47_08140)	pilU	1534078..1535358 (-)	1281	WP_003787551.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HBA47_RS08110 (HBA47_08145)	pilT	1535378..1536418 (-)	1041	WP_003791105.1	type IV pilus twitching motility protein PilT	Machinery gene
HBA47_RS08115 (HBA47_08150)	pseC	1536854..1538011 (-)	1158	WP_019389589.1	UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase	-
HBA47_RS08120 (HBA47_08155)	-	1538124..1539299 (-)	1176	WP_003787543.1	alpha-hydroxy acid oxidase	-

Sequence

Protein

• Length: 426 a.a.
• Molecular weight: 47321.06 Da
• Isoelectric Point: 6.2389

>NTDB_id=377065 HBA47_RS08105 WP_003787551.1 1534078..1535358(-) (pilU) [Kingella kingae strain ATCC 23332]
MAEQDITSLLEGFVNNYKVTNDAAKPAAPAGRAVPASAQATATASEIAPAKKSGLHPLLEKMAAECEKRGASDIFISAGF
PPSFKINGELTPVPLKPLTQQDTQILVYSTMSPDQRARFDHELELNYSVSSSEGIRFRVNSYHEQGRMGMVWRRITTKIL
TVDDLQLPQGLKDLAMQPRGLLILAGATGSGKSTSMAAMLDWRNKHAAGHIVTIEDPVEYLHKPIKSIFTQRELGVDTNS
WSMAVQSAMRQAPDVVCVGEVRNEHTMEYALQLAQTGHLCIFTIHANNAVQTIERIMNFYPEERHQQVLMDLALNTVGII
GQRLARLKGGGRTAIVDLLINSPAMQDHIFKGELMEAMSLMNRSETDGMQTFDQDLFRLYTEDKITYEEALRQAESVNDL
RLKIKLWEEGNAKDHIFERVGGLSLQ

Nucleotide

• Length: 1281 bp

>NTDB_id=377065 HBA47_RS08105 WP_003787551.1 1534078..1535358(-) (pilU) [Kingella kingae strain ATCC 23332]
ATGGCAGAACAAGACATCACTTCCCTACTTGAAGGTTTTGTAAACAATTACAAAGTAACCAACGATGCCGCCAAGCCTGC
TGCACCAGCAGGACGAGCCGTGCCTGCATCGGCACAAGCCACGGCAACGGCTTCGGAAATTGCGCCAGCCAAAAAATCGG
GCTTACACCCTTTGTTGGAAAAAATGGCTGCCGAATGTGAAAAACGCGGTGCATCGGATATTTTTATCAGCGCAGGTTTT
CCGCCATCATTCAAAATCAATGGTGAATTAACGCCTGTTCCGCTCAAACCGCTCACGCAGCAAGACACGCAAATTTTGGT
GTATTCCACCATGTCGCCAGACCAACGTGCGCGTTTTGACCACGAATTGGAATTGAACTACTCCGTGTCGTCTAGCGAGG
GCATTCGCTTTCGTGTGAACTCGTATCACGAGCAAGGTCGCATGGGCATGGTGTGGCGTCGCATCACCACCAAAATCTTA
ACGGTTGATGATTTGCAGTTGCCACAAGGTTTAAAAGACTTGGCGATGCAGCCGCGCGGTTTGTTGATTTTGGCAGGCGC
AACGGGTTCGGGTAAATCTACGTCTATGGCGGCGATGCTGGACTGGCGCAACAAACACGCCGCAGGTCATATTGTGACCA
TTGAAGACCCTGTCGAATATTTGCATAAGCCAATCAAGAGTATTTTCACGCAACGCGAATTGGGCGTGGATACGAATTCT
TGGTCAATGGCGGTGCAATCGGCAATGCGTCAAGCACCAGACGTGGTTTGTGTGGGTGAGGTGCGTAACGAACACACCAT
GGAATACGCTTTGCAACTGGCGCAAACAGGTCACTTGTGTATCTTTACCATTCACGCGAACAATGCCGTGCAAACCATTG
AGCGTATTATGAACTTCTATCCAGAAGAGCGTCATCAACAAGTATTAATGGACTTGGCGTTGAACACAGTGGGCATTATT
GGTCAGCGTTTGGCGCGATTGAAAGGCGGCGGACGTACGGCGATTGTGGATTTGTTGATTAACAGCCCTGCGATGCAAGA
CCACATTTTCAAAGGCGAGTTGATGGAAGCGATGTCGTTGATGAATCGTTCGGAAACAGACGGCATGCAAACGTTTGACC
AAGACTTGTTCCGCTTGTACACCGAAGACAAAATCACTTACGAAGAAGCCTTGCGCCAAGCTGAATCGGTAAACGACTTG
CGCTTGAAAATCAAATTGTGGGAAGAGGGTAATGCCAAAGACCACATCTTTGAGCGCGTGGGCGGTTTGAGCTTGCAATA
A

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (96-326)

3D structure

• AlphaFold DB: F5S877

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	47.696	86.62	0.413
pilU	Acinetobacter baylyi ADP1	49.711	81.221	0.404
pilU	Vibrio cholerae strain A1552	46.429	78.873	0.366