Detailed information NTG0376973

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HBA48_RS02645
• Genome accession: NZ_CP050135
• Coordinates: 499104..500384 (+)
• Length: 426 a.a.
• NCBI ID: WP_003787551.1
• Uniprot ID: F5S877
• Organism: Kingella kingae strain ATCC 23331
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 494104..505384

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBA48_RS02630 (HBA48_02650)	-	495392..496567 (+)	1176	WP_003787543.1	alpha-hydroxy acid oxidase	-
HBA48_RS02635 (HBA48_02655)	pseC	496680..497837 (+)	1158	WP_042925151.1	UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase	-
HBA48_RS02640 (HBA48_02660)	pilT	498044..499084 (+)	1041	WP_003791105.1	type IV pilus twitching motility protein PilT	Machinery gene
HBA48_RS02645 (HBA48_02665)	pilU	499104..500384 (+)	1281	WP_003787551.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HBA48_RS02650 (HBA48_02670)	-	500463..500906 (+)	444	WP_038307164.1	peroxiredoxin	-
HBA48_RS02655 (HBA48_02675)	-	500903..501280 (+)	378	WP_166503186.1	diacylglycerol kinase	-
HBA48_RS02660 (HBA48_02680)	-	501351..501860 (-)	510	WP_003791099.1	gluconokinase	-
HBA48_RS02665 (HBA48_02685)	-	501985..503358 (-)	1374	WP_003791097.1	GntP family permease	-
HBA48_RS02670 (HBA48_02690)	folD	503501..504352 (-)	852	WP_003791096.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-

Sequence

Protein

• Length: 426 a.a.
• Molecular weight: 47321.06 Da
• Isoelectric Point: 6.2389

>NTDB_id=376973 HBA48_RS02645 WP_003787551.1 499104..500384(+) (pilU) [Kingella kingae strain ATCC 23331]
MAEQDITSLLEGFVNNYKVTNDAAKPAAPAGRAVPASAQATATASEIAPAKKSGLHPLLEKMAAECEKRGASDIFISAGF
PPSFKINGELTPVPLKPLTQQDTQILVYSTMSPDQRARFDHELELNYSVSSSEGIRFRVNSYHEQGRMGMVWRRITTKIL
TVDDLQLPQGLKDLAMQPRGLLILAGATGSGKSTSMAAMLDWRNKHAAGHIVTIEDPVEYLHKPIKSIFTQRELGVDTNS
WSMAVQSAMRQAPDVVCVGEVRNEHTMEYALQLAQTGHLCIFTIHANNAVQTIERIMNFYPEERHQQVLMDLALNTVGII
GQRLARLKGGGRTAIVDLLINSPAMQDHIFKGELMEAMSLMNRSETDGMQTFDQDLFRLYTEDKITYEEALRQAESVNDL
RLKIKLWEEGNAKDHIFERVGGLSLQ

Nucleotide

• Length: 1281 bp

>NTDB_id=376973 HBA48_RS02645 WP_003787551.1 499104..500384(+) (pilU) [Kingella kingae strain ATCC 23331]
ATGGCAGAACAAGACATCACTTCCCTACTTGAAGGTTTTGTAAATAATTACAAAGTAACCAACGATGCCGCCAAGCCTGC
TGCACCAGCAGGACGAGCCGTGCCTGCATCGGCACAAGCCACGGCAACGGCTTCGGAAATTGCGCCAGCCAAAAAATCGG
GCTTACACCCTTTGTTGGAAAAAATGGCTGCCGAATGTGAAAAACGCGGTGCATCGGATATTTTTATCAGCGCAGGTTTT
CCGCCATCATTCAAAATCAATGGCGAATTAACGCCTGTTCCGCTCAAACCGCTCACGCAGCAAGACACGCAAATTTTGGT
GTATTCCACCATGTCGCCAGACCAACGTGCGCGTTTTGACCACGAGCTAGAATTGAACTACTCCGTGTCGTCTAGCGAGG
GTATTCGCTTTCGTGTGAACTCGTATCACGAGCAAGGTCGCATGGGCATGGTGTGGCGTCGCATCACCACCAAAATCTTA
ACGGTTGATGATTTGCAGTTGCCACAAGGTTTAAAAGACTTGGCGATGCAGCCGCGCGGTTTGTTGATTTTGGCAGGCGC
AACGGGTTCGGGTAAATCTACGTCTATGGCGGCGATGCTGGACTGGCGCAACAAACACGCCGCAGGTCATATTGTGACCA
TTGAAGACCCTGTCGAATATTTGCATAAGCCAATCAAGAGTATTTTCACGCAACGCGAATTGGGCGTGGATACGAATTCT
TGGTCAATGGCGGTGCAATCGGCAATGCGTCAAGCACCAGACGTGGTTTGTGTGGGTGAGGTGCGTAACGAACACACCAT
GGAATACGCTTTGCAACTGGCGCAAACAGGTCACTTGTGTATCTTTACTATTCACGCGAACAATGCCGTGCAAACCATTG
AGCGTATTATGAACTTCTATCCAGAAGAACGTCATCAACAAGTATTGATGGACTTGGCGTTGAACACAGTGGGCATTATT
GGTCAGCGTTTGGCGCGATTGAAAGGCGGCGGACGTACGGCGATTGTGGATTTGTTGATTAACAGCCCTGCGATGCAAGA
CCACATTTTCAAAGGCGAGTTGATGGAAGCGATGTCGTTGATGAATCGTTCGGAAACAGACGGCATGCAAACGTTTGACC
AAGACTTGTTCCGCTTGTACACCGAAGACAAAATCACTTACGAAGAAGCCTTGCGCCAAGCTGAATCGGTAAACGACTTG
CGCTTGAAAATCAAATTGTGGGAAGAGGGTAATGCCAAAGACCACATCTTTGAGCGCGTGGGCGGTTTGAGCTTGCAATA
A

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (96-326)

3D structure

• AlphaFold DB: F5S877

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	47.696	86.62	0.413
pilU	Acinetobacter baylyi ADP1	49.711	81.221	0.404
pilU	Vibrio cholerae strain A1552	46.429	78.873	0.366