Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   G9W35_RS08490 Genome accession   NZ_CP049845
Coordinates   1762311..1762970 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain UPEC-245U/190328     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1757311..1767970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9W35_RS08465 (G9W35_08405) yqiA 1757379..1757960 (+) 582 WP_000105733.1 esterase YqiA -
  G9W35_RS08470 (G9W35_08410) parE 1757989..1759881 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  G9W35_RS08475 (G9W35_08415) ygiN 1759929..1760243 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  G9W35_RS08480 (G9W35_08420) mdaB 1760274..1760855 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  G9W35_RS08485 (G9W35_08425) qseC 1760965..1762314 (-) 1350 WP_023140511.1 quorum sensing histidine kinase QseC -
  G9W35_RS08490 (G9W35_08430) ciaR 1762311..1762970 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  G9W35_RS08495 (G9W35_08435) ygiW 1763122..1763514 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  G9W35_RS08500 (G9W35_08440) ygiV 1763567..1764049 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  G9W35_RS08505 (G9W35_08445) parC 1764595..1766853 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  G9W35_RS08510 (G9W35_08450) plsC 1767087..1767824 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=375438 G9W35_RS08490 WP_001221502.1 1762311..1762970(-) (ciaR) [Escherichia coli strain UPEC-245U/190328]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=375438 G9W35_RS08490 WP_001221502.1 1762311..1762970(-) (ciaR) [Escherichia coli strain UPEC-245U/190328]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment