Detailed information NTG0375374

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: G9W36_RS19485
• Genome accession: NZ_CP049839
• Coordinates: 4016156..4016815 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain UPEC-455U/190220
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 4011156..4021815

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G9W36_RS19460 (G9W36_19435)	ygiS	4012439..4014046 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
G9W36_RS19465 (G9W36_19440)	mqsA	4014179..4014574 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
G9W36_RS19470 (G9W36_19445)	mqsR	4014576..4014872 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
G9W36_RS19475 (G9W36_19450)	ygiV	4015077..4015559 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
G9W36_RS19480 (G9W36_19455)	ygiW	4015612..4016004 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
G9W36_RS19485 (G9W36_19460)	ciaR	4016156..4016815 (+)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
G9W36_RS19490 (G9W36_19465)	qseC	4016812..4018161 (+)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
G9W36_RS19495 (G9W36_19470)	ygiZ	4018207..4018539 (-)	333	WP_000917685.1	DUF2645 family protein	-
G9W36_RS19500 (G9W36_19475)	mdaB	4018858..4019439 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
G9W36_RS19505 (G9W36_19480)	ygiN	4019470..4019784 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
G9W36_RS19510 (G9W36_19485)	parE	4019832..4021724 (-)	1893	WP_219891818.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=375374 G9W36_RS19485 WP_001221493.1 4016156..4016815(+) (ciaR) [Escherichia coli strain UPEC-455U/190220]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=375374 G9W36_RS19485 WP_001221493.1 4016156..4016815(+) (ciaR) [Escherichia coli strain UPEC-455U/190220]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365