Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FOV14_RS11860 Genome accession   NZ_CP041784
Coordinates   2478919..2479989 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain IM1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2473919..2484989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOV14_RS11810 (FOV14_11885) - 2473979..2474563 (-) 585 WP_240679749.1 signal peptidase I -
  FOV14_RS11815 (FOV14_11890) tapA 2474535..2475206 (-) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  FOV14_RS11820 (FOV14_11895) - 2475465..2475794 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  FOV14_RS11825 (FOV14_11900) - 2475834..2476013 (-) 180 WP_003153093.1 YqzE family protein -
  FOV14_RS11830 (FOV14_11905) comGG 2476070..2476447 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  FOV14_RS11835 (FOV14_11910) comGF 2476448..2476948 (-) 501 WP_235570156.1 competence type IV pilus minor pilin ComGF -
  FOV14_RS11840 (FOV14_11915) comGE 2476857..2477171 (-) 315 WP_057080485.1 competence type IV pilus minor pilin ComGE Machinery gene
  FOV14_RS11845 (FOV14_11920) comGD 2477155..2477592 (-) 438 WP_015388002.1 competence type IV pilus minor pilin ComGD Machinery gene
  FOV14_RS11850 (FOV14_11925) comGC 2477582..2477848 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  FOV14_RS11855 (FOV14_11930) comGB 2477895..2478932 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  FOV14_RS11860 (FOV14_11935) comGA 2478919..2479989 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  FOV14_RS11865 (FOV14_11940) - 2480181..2481131 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  FOV14_RS11870 (FOV14_11945) - 2481277..2482578 (+) 1302 WP_014305416.1 hemolysin family protein -
  FOV14_RS11875 (FOV14_11950) - 2482616..2483446 (-) 831 WP_003153080.1 STAS domain-containing protein -
  FOV14_RS11885 (FOV14_11960) - 2483652..2484032 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FOV14_RS11890 (FOV14_11965) - 2484269..2484514 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=374306 FOV14_RS11860 WP_003153083.1 2478919..2479989(-) (comGA) [Bacillus velezensis strain IM1]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=374306 FOV14_RS11860 WP_003153083.1 2478919..2479989(-) (comGA) [Bacillus velezensis strain IM1]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment