Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M301_RS10365 Genome accession   NC_014207
Coordinates   2277504..2278505 (+) Length   333 a.a.
NCBI ID   WP_013148730.1    Uniprot ID   -
Organism   Methylotenera versatilis 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2272504..2283505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS10345 (M301_2042) - 2273581..2274906 (-) 1326 WP_081439437.1 M16 family metallopeptidase -
  M301_RS10350 (M301_2043) - 2275215..2276462 (+) 1248 WP_013148727.1 OmpP1/FadL family transporter -
  M301_RS10355 (M301_2044) - 2276540..2276971 (+) 432 WP_013148728.1 YeeE/YedE family protein -
  M301_RS10360 (M301_2045) - 2276980..2277408 (+) 429 WP_013148729.1 DUF6691 family protein -
  M301_RS10365 (M301_2046) pilA 2277504..2278505 (+) 1002 WP_013148730.1 signal recognition particle-docking protein FtsY Machinery gene
  M301_RS10370 (M301_2047) ftsE 2278542..2279228 (+) 687 WP_013148731.1 cell division ATP-binding protein FtsE -
  M301_RS10375 (M301_2048) ftsX 2279221..2280141 (+) 921 WP_013148732.1 permease-like cell division protein FtsX -
  M301_RS10380 (M301_2049) - 2280363..2280755 (+) 393 WP_013148733.1 CZB domain-containing protein -
  M301_RS10385 (M301_2050) - 2280933..2281244 (+) 312 WP_013148734.1 hypothetical protein -
  M301_RS14600 (M301_2051) - 2281766..2281939 (+) 174 WP_013148735.1 hypothetical protein -
  M301_RS10390 (M301_2053) - 2282333..2283124 (+) 792 WP_013148737.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 35686.90 Da        Isoelectric Point: 5.2515

>NTDB_id=37393 M301_RS10365 WP_013148730.1 2277504..2278505(+) (pilA) [Methylotenera versatilis 301]
MFDFFKKKPFQPEHEETTEQVDTALTEAQVAAQSWSERLKQSLSKTRKQLGNQLASLFGGGKIDAEVYEELETILITSDV
GVNATKNLLADIKNRVKVQSLDDTVQLKAALKEAMSDLLTPLQKPLDTSGQKPFVIMLAGVNGAGKTTSIGKLAHAFQAE
GKSVLIAAGDTFRAAAREQLQIWGERNNVHVVSQTSGDAAAVMFDAINSAKAKNIDIVLADTAGRLPTQMHLMDEIAKVK
RVMDKALPGAPHEVLLVLDANTGQNAVTQVKTFDDALGVTGLVLSKLDGTAKGGVIAAIAEARPIPIRYIGIGEGIDDLR
PFIAADFIDAMID

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=37393 M301_RS10365 WP_013148730.1 2277504..2278505(+) (pilA) [Methylotenera versatilis 301]
ATGTTCGATTTTTTCAAAAAAAAACCATTTCAGCCTGAGCACGAAGAAACTACTGAGCAAGTAGATACTGCACTCACCGA
AGCGCAAGTAGCCGCACAAAGCTGGAGTGAGCGCCTAAAACAAAGCCTCAGCAAAACACGTAAACAGCTCGGCAATCAAC
TTGCCAGTTTGTTTGGTGGCGGCAAAATCGACGCAGAAGTTTATGAAGAGCTAGAAACTATTTTAATCACTTCAGATGTA
GGCGTGAATGCTACTAAAAACCTGCTTGCCGACATTAAAAACCGCGTAAAAGTGCAAAGTCTAGATGATACAGTTCAGCT
TAAAGCCGCGCTAAAAGAAGCTATGTCTGACTTACTTACGCCACTACAAAAGCCGCTCGATACCAGCGGCCAAAAGCCCT
TTGTGATTATGCTAGCAGGGGTGAATGGCGCAGGTAAAACTACGTCAATTGGCAAGCTTGCGCATGCATTTCAAGCAGAA
GGTAAGTCTGTACTCATTGCCGCAGGTGATACATTTAGAGCTGCCGCACGCGAACAACTGCAAATTTGGGGAGAACGCAA
TAACGTACATGTCGTTTCGCAAACCAGTGGTGACGCGGCTGCCGTGATGTTTGATGCCATCAACTCAGCAAAAGCTAAGA
ATATCGACATCGTACTAGCAGACACTGCAGGCCGCTTGCCTACGCAAATGCACTTAATGGATGAAATTGCCAAAGTTAAA
CGCGTGATGGATAAAGCCCTGCCTGGTGCACCTCACGAAGTGTTATTGGTGCTAGACGCTAACACTGGCCAAAATGCCGT
GACGCAAGTAAAAACCTTCGACGACGCACTAGGTGTTACAGGCTTGGTTCTTAGTAAGCTAGATGGTACCGCTAAAGGCG
GCGTCATTGCGGCGATTGCTGAAGCACGCCCTATCCCAATTCGTTACATTGGCATCGGCGAAGGCATAGACGATTTACGA
CCGTTTATTGCTGCGGATTTTATTGATGCAATGATTGATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(49-115)

SRP54-type protein, GTPase domain (SRP54)

(133-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

64.577

95.796

0.619

Multiple sequence alignment