Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FFH21_RS04175 Genome accession   NZ_CP041754
Coordinates   894217..895089 (-) Length   290 a.a.
NCBI ID   WP_011167726.1    Uniprot ID   Q48NC1
Organism   Pseudomonas sp. KBS0707     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 889217..900089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFH21_RS04165 (FFH21_004165) yacG 893391..893600 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  FFH21_RS04170 (FFH21_004170) coaE 893597..894220 (-) 624 WP_011167725.1 dephospho-CoA kinase -
  FFH21_RS04175 (FFH21_004175) pilD 894217..895089 (-) 873 WP_011167726.1 A24 family peptidase Machinery gene
  FFH21_RS04180 (FFH21_004180) pilC 895091..896308 (-) 1218 WP_011167727.1 type II secretion system F family protein Machinery gene
  FFH21_RS04185 (FFH21_004185) pilB 896311..898005 (-) 1695 WP_011167728.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FFH21_RS04190 (FFH21_004190) - 898230..898625 (+) 396 WP_011167729.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32059.31 Da        Isoelectric Point: 8.7845

>NTDB_id=373754 FFH21_RS04175 WP_011167726.1 894217..895089(-) (pilD) [Pseudomonas sp. KBS0707]
MPLLDLLASSPLAFVITCCILGLIIGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPRCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYIAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide


Download         Length: 873 bp        

>NTDB_id=373754 FFH21_RS04175 WP_011167726.1 894217..895089(-) (pilD) [Pseudomonas sp. KBS0707]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCACCGCTGGCCTTCGTCATTACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCCAAAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAAATGCTCGGCC
TGCCCGCGGAACCCGATCAGCCCGCCTTCAACCTGAACCGCCCTCGCTCCAGCTGCCCGCGCTGCGCGCACAAAATCCGC
CCGTGGGAAAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCACAATGCAAAGCCCCGATCAGCAAACG
CTACCCATTGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACATCGCCTGGCATTTCGGCTTCGGCTGGCAGGCAGCGG
CGATGCTGGTGTTGAGCTGGGGCCTGCTGGCGATGAGTCTGATCGACGCCGATCACCAGCTGTTACCTGACTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTAAACGCTTTCGGGCTGTTCACCTCGCTGAACGATGCGCTGTGGGGCGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTATTCAAACTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCATCGCTGGTGGGT
GCGGTGCTTGGGGTGATCATGATGCGCGTGCGCAGGGTTGAAAGTGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(136-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q48NC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.521

97.931

0.524
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter baumannii D1279779

53.285

94.483

0.503
  pilD Acinetobacter nosocomialis M2

52.19

94.483

0.493
  pilD Neisseria gonorrhoeae MS11

51.119

92.414

0.472

Multiple sequence alignment