Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   G5T40_RS00750 Genome accession   NZ_CP049196
Coordinates   151648..152307 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain E118     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 146648..157307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5T40_RS00725 parE 146739..148631 (+) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -
  G5T40_RS00730 ygiN 148679..148993 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  G5T40_RS00735 mdaB 149024..149605 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  G5T40_RS00740 ygiZ 149924..150256 (+) 333 WP_023149630.1 DUF2645 family protein -
  G5T40_RS00745 qseC 150302..151651 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  G5T40_RS00750 ciaR 151648..152307 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  G5T40_RS00755 ygiW 152459..152851 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  G5T40_RS00760 ygiV 152904..153386 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  G5T40_RS00765 mqsR 153591..153887 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  G5T40_RS00770 mqsA 153889..154284 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  G5T40_RS00775 ygiS 154417..156024 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=372890 G5T40_RS00750 WP_001221493.1 151648..152307(-) (ciaR) [Escherichia coli strain E118]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=372890 G5T40_RS00750 WP_001221493.1 151648..152307(-) (ciaR) [Escherichia coli strain E118]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365