Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   G5V60_RS03580 Genome accession   NZ_CP049101
Coordinates   735147..735806 (-) Length   219 a.a.
NCBI ID   WP_001525668.1    Uniprot ID   -
Organism   Escherichia coli strain EC28     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 730147..740806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS03555 (G5V60_03555) ygiN 731048..731362 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  G5V60_RS03560 (G5V60_03560) mdaB 731393..731974 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  G5V60_RS25925 - 732224..732703 (+) 480 WP_000065328.1 Hcp family type VI secretion system effector -
  G5V60_RS25930 - 732706..733416 (+) 711 WP_001525672.1 hypothetical protein -
  G5V60_RS03570 (G5V60_03570) ygiZ 733423..733755 (+) 333 WP_000914696.1 DUF2645 family protein -
  G5V60_RS03575 (G5V60_03575) qseC 733801..735150 (-) 1350 WP_001525670.1 quorum sensing histidine kinase QseC -
  G5V60_RS03580 (G5V60_03580) ciaR 735147..735806 (-) 660 WP_001525668.1 quorum sensing response regulator transcription factor QseB Regulator
  G5V60_RS03585 (G5V60_03585) ygiW 735958..736350 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  G5V60_RS03590 (G5V60_03590) ygiV 736403..736885 (+) 483 WP_000183484.1 GyrI-like domain-containing protein -
  G5V60_RS03595 (G5V60_03595) ygiS 736994..738601 (+) 1608 WP_001525667.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24673.60 Da        Isoelectric Point: 6.9850

>NTDB_id=372384 G5V60_RS03580 WP_001525668.1 735147..735806(-) (ciaR) [Escherichia coli strain EC28]
MRVLLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=372384 G5V60_RS03580 WP_001525668.1 735147..735806(-) (ciaR) [Escherichia coli strain EC28]
ATGCGAGTTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.874

100

0.365