Detailed information NTG0370744

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: QE11421_RS10960
• Genome accession: NZ_CP048821
• Coordinates: 2228796..2229455 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain QEC11-421
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 2223796..2234455

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QE11421_RS10935 (QE11421_10590)	ygiS	2225079..2226686 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
QE11421_RS10940 (QE11421_10595)	mqsA	2226819..2227214 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
QE11421_RS10945 (QE11421_10600)	mqsR	2227216..2227512 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
QE11421_RS10950 (QE11421_10605)	ygiV	2227717..2228199 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
QE11421_RS10955 (QE11421_10610)	ygiW	2228252..2228644 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
QE11421_RS10960 (QE11421_10615)	ciaR	2228796..2229455 (+)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
QE11421_RS10965 (QE11421_10620)	qseC	2229452..2230801 (+)	1350	WP_001618857.1	quorum sensing histidine kinase QseC	-
QE11421_RS10970	ygiZ	2230847..2231179 (-)	333	WP_000917684.1	DUF2645 family protein	-
QE11421_RS10975 (QE11421_10625)	mdaB	2231498..2232079 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
QE11421_RS10980 (QE11421_10630)	ygiN	2232110..2232424 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
QE11421_RS10985 (QE11421_10635)	parE	2232472..2234364 (-)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=370744 QE11421_RS10960 WP_001221493.1 2228796..2229455(+) (ciaR) [Escherichia coli strain QEC11-421]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=370744 QE11421_RS10960 WP_001221493.1 2228796..2229455(+) (ciaR) [Escherichia coli strain QEC11-421]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365