Detailed information NTG0370380

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BvL003_RS12040
• Genome accession: NZ_CP041192
• Coordinates: 2503040..2503654 (-)
• Length: 204 a.a.
• NCBI ID: WP_146277193.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain BvL03
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2498040..2508654

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BvL003_RS12010 (BvL003_12165)	rpsT	2498340..2498606 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BvL003_RS12015 (BvL003_12170)	holA	2498623..2499664 (-)	1042	Protein_2336	DNA polymerase III subunit delta	-
BvL003_RS12020 (BvL003_12175)	-	2499704..2499858 (-)	155	Protein_2337	hypothetical protein	-
BvL003_RS12025 (BvL003_12180)	-	2499899..2500033 (+)	135	WP_003152870.1	YqzM family protein	-
BvL003_RS12030 (BvL003_12185)	comEC	2500052..2502403 (-)	2352	WP_146277191.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BvL003_RS12035 (BvL003_12190)	-	2502404..2502973 (-)	570	WP_003152868.1	ComE operon protein 2	-
BvL003_RS12040 (BvL003_12195)	comEA	2503040..2503654 (-)	615	WP_146277193.1	helix-hairpin-helix domain-containing protein	Machinery gene
BvL003_RS12045 (BvL003_12200)	comER	2503713..2504534 (+)	822	WP_146277195.1	late competence protein ComER	-
BvL003_RS12050 (BvL003_12205)	-	2504603..2505340 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
BvL003_RS12055 (BvL003_12210)	rsfS	2505337..2505693 (-)	357	WP_003152864.1	ribosome silencing factor	-
BvL003_RS12060 (BvL003_12215)	yqeK	2505711..2506271 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BvL003_RS12065 (BvL003_12220)	-	2506261..2506830 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
BvL003_RS12070 (BvL003_12225)	yhbY	2506841..2507131 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BvL003_RS12075 (BvL003_12230)	aroE	2507125..2507967 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22218.33 Da
• Isoelectric Point: 6.0523

>NTDB_id=370380 BvL003_RS12040 WP_146277193.1 2503040..2503654(-) (comEA) [Bacillus velezensis strain BvL03]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLRDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=370380 BvL003_RS12040 WP_146277193.1 2503040..2503654(-) (comEA) [Bacillus velezensis strain BvL03]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCGGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363