Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GCO85_RS06710 Genome accession   NZ_CP048618
Coordinates   1471857..1473341 (-) Length   494 a.a.
NCBI ID   WP_061636319.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466857..1478341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS06695 (GCO85_06705) cysS 1467850..1469220 (+) 1371 WP_061636318.1 cysteine--tRNA ligase -
  GCO85_RS06700 (GCO85_06710) - 1469399..1471312 (+) 1914 WP_011213619.1 DUF3413 domain-containing protein -
  GCO85_RS06705 (GCO85_06715) - 1471442..1471756 (+) 315 WP_011213620.1 DUF6482 family protein -
  GCO85_RS06710 (GCO85_06720) pilB 1471857..1473341 (-) 1485 WP_061636319.1 GspE family T2SS ATPase variant LspE Machinery gene
  GCO85_RS06715 (GCO85_06725) lspD 1473345..1475720 (-) 2376 WP_011213622.1 GspD family T2SS secretin variant LspD -
  GCO85_RS06720 (GCO85_06730) - 1475893..1476921 (+) 1029 WP_027220708.1 Gfo/Idh/MocA family oxidoreductase -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55149.46 Da        Isoelectric Point: 5.4872

>NTDB_id=370095 GCO85_RS06710 WP_061636319.1 1471857..1473341(-) (pilB) [Legionella pneumophila strain ERS1305867]
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPATPSIREDGFRRVLCG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=370095 GCO85_RS06710 WP_061636319.1 1471857..1473341(-) (pilB) [Legionella pneumophila strain ERS1305867]
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACAGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATACAAGA
AAATCTGGCTATTGTCTATCACTTACCTAACACACCACTGCAAGCTTTCGCTGAGATAAAGCGATTATTACAATGTGAAC
TTGATCTAAAACAAGTCGATGAATCCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCTGCAGAAGGCATGGAAGAAGATATGGATTTATCCATGTTGGCCAGTCAACTTCCCGTCAGTGAAGACTTATTGGAAAA
CCAAGACGATGCCCCAATTATCCGCTTACTGAACGCTCTGTTTACCCAAGCGATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTGCGCAATCGAATTGATGGTGTTCTACAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGAATTCCTCAAGATGGTCG
AATTTCATTGCGTATAGGAGGACATAATATTGATGTCAGGGTCTCAACTTTACCCTCAAACCATGGAGAACGAGTGGTTT
TAAGGATATTGGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTGAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCCCATGGCATTATTTTAGTCACTGGCCCAACAGGTTCAGGCAAGACAACATCTCTTTATGCTAT
GCTCACCGAACTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACCTGCCTGGTGTAGGAC
AAACGCAGGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCCATATTACGTCAAGATCCGGATGTTGTC
ATGATAGGAGAAATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCCAGCTTAACAGGGCATTTGGTACTATCAAC
CCTTCATACCAATACCGCATTAGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGCCTCATTGCGCAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCTCATGAATTAAGGCAGGAAGAA
AAGGAGTTAATGGGTCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAGCCCAAGGGATGCGACTATTGCAACCACTT
GGGTTATAAAGGCAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACAATGGAAAATTATTTACGACCCGCCACGCCGAGCATACGTGAAGATGGTTTTAGAAGAGTATTATGTGGC
GATACGTCACTTGCAGAAATTTTAAGGGTAACATCCCAAAATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.067

96.356

0.415
  pilB Vibrio campbellii strain DS40M4

46.305

82.186

0.381
  pilB Acinetobacter baumannii D1279779

44.712

84.211

0.377
  pilB Vibrio parahaemolyticus RIMD 2210633

46.04

81.781

0.377
  pilB Acinetobacter baylyi ADP1

47.301

78.745

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

42.13

87.449

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.524

79.15

0.36