Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   D073_RS16685 Genome accession   NZ_CP041145
Coordinates   3365576..3366961 (-) Length   461 a.a.
NCBI ID   WP_020956417.1    Uniprot ID   -
Organism   Bacillus velezensis strain At1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360576..3371961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D073_RS16650 (D073_3154) flgL 3360751..3361668 (-) 918 WP_020956415.1 flagellar hook-associated protein FlgL -
  D073_RS16655 (D073_3155) flgK 3361680..3363197 (-) 1518 WP_007407478.1 flagellar hook-associated protein FlgK -
  D073_RS16660 (D073_3156) - 3363213..3363695 (-) 483 WP_003151394.1 flagellar protein FlgN -
  D073_RS16665 (D073_3157) flgM 3363710..3363976 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  D073_RS16670 (D073_3158) - 3364046..3364465 (-) 420 WP_007407479.1 TIGR03826 family flagellar region protein -
  D073_RS16675 comFC 3364539..3365228 (-) 690 WP_197538283.1 phosphoribosyltransferase family protein Machinery gene
  D073_RS16680 (D073_3160) - 3365234..3365518 (-) 285 WP_007407481.1 late competence development ComFB family protein -
  D073_RS16685 (D073_3161) comFA 3365576..3366961 (-) 1386 WP_020956417.1 DEAD/DEAH box helicase Machinery gene
  D073_RS16690 (D073_3162) - 3367070..3367918 (-) 849 WP_007407483.1 DegV family protein -
  D073_RS16695 (D073_3163) degU 3368016..3368705 (-) 690 WP_020956418.1 two-component system response regulator DegU Regulator
  D073_RS16700 (D073_3164) degS 3368782..3369945 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  D073_RS16705 (D073_3165) - 3370168..3370815 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52753.12 Da        Isoelectric Point: 10.2027

>NTDB_id=369850 D073_RS16685 WP_020956417.1 3365576..3366961(-) (comFA) [Bacillus velezensis strain At1]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITDEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYYWNQAHENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=369850 D073_RS16685 WP_020956417.1 3365576..3366961(-) (comFA) [Bacillus velezensis strain At1]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTTACAGAAAAAGAATATATAACGGACGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTATTGGAATCAAGCCCATGAAAACAG
CTGGGAACCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACCCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTTCATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(328-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62

Multiple sequence alignment