Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   G3M71_RS03440 Genome accession   NZ_CP048603
Coordinates   715459..716118 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain ChlosBP-23-1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 710459..721118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M71_RS03415 (G3M71_03415) - 711201..712148 (+) 948 WP_001296418.1 iron-siderophore ABC transporter substrate-binding protein -
  G3M71_RS03420 (G3M71_03420) - 712145..713032 (+) 888 WP_000614943.1 MurR/RpiR family transcriptional regulator -
  G3M71_RS03425 (G3M71_03425) ygiN 713077..713391 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  G3M71_RS03430 (G3M71_03430) mdaB 713422..714003 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  G3M71_RS03435 (G3M71_03435) qseC 714113..715462 (-) 1350 WP_021529017.1 quorum sensing histidine kinase QseC -
  G3M71_RS03440 (G3M71_03440) ciaR 715459..716118 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  G3M71_RS03445 (G3M71_03445) ygiW 716270..716662 (+) 393 WP_021529016.1 OB fold stress tolerance protein YgiW -
  G3M71_RS03450 (G3M71_03450) ygiV 716715..717197 (+) 483 WP_000183493.1 GyrI-like domain-containing protein -
  G3M71_RS03455 (G3M71_03455) ygiS 717306..718913 (+) 1608 WP_021529015.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=369770 G3M71_RS03440 WP_001221493.1 715459..716118(-) (ciaR) [Escherichia coli strain ChlosBP-23-1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=369770 G3M71_RS03440 WP_001221493.1 715459..716118(-) (ciaR) [Escherichia coli strain ChlosBP-23-1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment