Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FIM06_RS11995 Genome accession   NZ_CP041144
Coordinates   2458243..2459313 (-) Length   356 a.a.
NCBI ID   WP_007612574.1    Uniprot ID   -
Organism   Bacillus velezensis strain UCMB5044     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2453243..2464313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIM06_RS11945 (FIM06_2226) sipW 2453302..2453886 (-) 585 WP_015240205.1 signal peptidase I SipW -
  FIM06_RS11950 (FIM06_2227) tapA 2453858..2454529 (-) 672 WP_044802562.1 amyloid fiber anchoring/assembly protein TapA -
  FIM06_RS11955 (FIM06_2228) - 2454788..2455117 (+) 330 WP_038459175.1 DUF3889 domain-containing protein -
  FIM06_RS11960 (FIM06_2229) - 2455158..2455337 (-) 180 WP_022552966.1 YqzE family protein -
  FIM06_RS11965 (FIM06_2230) comGG 2455394..2455771 (-) 378 WP_038459177.1 competence type IV pilus minor pilin ComGG Machinery gene
  FIM06_RS11970 (FIM06_2231) comGF 2455772..2456272 (-) 501 WP_228842201.1 competence type IV pilus minor pilin ComGF -
  FIM06_RS11975 (FIM06_2232) comGE 2456181..2456495 (-) 315 WP_105937524.1 competence type IV pilus minor pilin ComGE -
  FIM06_RS11980 (FIM06_2233) comGD 2456479..2456916 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  FIM06_RS11985 (FIM06_2234) comGC 2456906..2457214 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  FIM06_RS11990 (FIM06_2235) comGB 2457219..2458256 (-) 1038 WP_105937523.1 competence type IV pilus assembly protein ComGB Machinery gene
  FIM06_RS11995 (FIM06_2236) comGA 2458243..2459313 (-) 1071 WP_007612574.1 competence type IV pilus ATPase ComGA Machinery gene
  FIM06_RS12000 (FIM06_2237) - 2459511..2460461 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  FIM06_RS12005 (FIM06_2238) - 2460607..2461908 (+) 1302 WP_105937521.1 hemolysin family protein -
  FIM06_RS12010 (FIM06_2239) - 2461946..2462776 (-) 831 WP_029326073.1 STAS domain-containing protein -
  FIM06_RS12020 (FIM06_2241) - 2462982..2463362 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  FIM06_RS12025 (FIM06_2242) - 2463599..2463844 (+) 246 WP_105937520.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.84 Da        Isoelectric Point: 9.3437

>NTDB_id=369756 FIM06_RS11995 WP_007612574.1 2458243..2459313(-) (comGA) [Bacillus velezensis strain UCMB5044]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRETRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=369756 FIM06_RS11995 WP_007612574.1 2458243..2459313(-) (comGA) [Bacillus velezensis strain UCMB5044]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTCGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGGAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

38.177

98.596

0.376

Multiple sequence alignment